Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 265-512-0 | CAS number: 65140-91-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- endocrine system modulation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards, well documented and acceptable for assessment. No data on purity were available.
Data source
Reference
- Reference Type:
- publication
- Title:
- Estrogenic activities of chemicals related to food contact plastics and rubbers tested by the yeast two-hybrid assay.
- Author:
- Ogawa Y et al.
- Year:
- 2 006
- Bibliographic source:
- Food Additives and Contaminants (23) 4: 422-430.
Materials and methods
- Principles of method if other than guideline:
- A yeast two-hybrid assay was performed. The results were evaluated on the basis of the relative activity, expressed as 10% relative effective concentration (REC10), which is the concentration of the test chemical showing 10% of the agonist activity of 10xE-6 mol/L 17-beta-Estradiol, the highest activity level of 17-beta-Estradiol.
- GLP compliance:
- no
- Type of method:
- in vitro
- Endpoint addressed:
- other: estrogenic activity
Test material
- Reference substance name:
- Calcium diethyl bis[[[3,5-bis(1,1-dimethylethyl)-4-hydroxyphenyl]methyl]phosphonate]
- EC Number:
- 265-512-0
- EC Name:
- Calcium diethyl bis[[[3,5-bis(1,1-dimethylethyl)-4-hydroxyphenyl]methyl]phosphonate]
- Cas Number:
- 65140-91-2
- Molecular formula:
- C17 H29 O4 P. 1/2Ca
- IUPAC Name:
- calcium diethyl bis[[[3,5-bis(1,1-dimethylethyl)-4-hydroxyphenyl]methyl]phosphonate]
- Details on test material:
- Purity: No data
Constituent 1
Administration / exposure
- Details on study design:
- - The yeast two-hybrid cells were preincubated overnight at 30°C with vigorous shaking in a SD medium which was free from tryptophan and leucine. The culture was diluted with 4 volumes of the fresh SD medium and 250 µl of this solution put into a small test tube. The test chemical solution (2.5 µl) was added and incubated for 4 h at 30° C. After incubation, 150 µl of the culture solution was placed into each of the 96 wells of a microplate and the absorbancy measured at 595 nm. The rest of the culture was centrifuged at 10,000 rpm for 7 min, after which the supernatant was removed. The cells were enzymatically digested by incubation with 4mg/mL Zymolyase 20T (200 µl) at 30°C for 15 min. The cell lysate was mixed with 4 mg/mLI ONPG (40 µl) and incubated at 30°C for exactly 30 min. The reaction was stopped by the addition of 1 mol/L Na2CO3. After centrifugation at 10000 rpm for 5 min, the supernatant (150 µL) was placed into each well of a microplate. The absorbances at 420 and 570 nm were read using a microplate reader. The ß-galactosidase activity was calculated using the following equation: U=1000 x ([OD420] - [1.75 x OD570])/([t] x [v] x [OD595]), where t = time of reaction (min), v = volume of culture used in the assay (mL), OD595 =cell density at the start of the assay, OD420 = absorbance by o-nitrophenol at the end of the reaction, and OD570 = light scattering at the end of the reaction. The ß-galactosidase activity was expressed as the mean and standard deviation of the results from three separate test tubes.
- Preparation of metabolites and their measurement of estrogenic activity:
To a tube containing 990 µl of the S9-mix, 10 µL of the test chemical solution (mainly 10xE-1 to 10xE-5 mol/L) was added, incubated at 37°C for 4h and then stored at -80°C until the yeast two-hybrid test was run as metabolite solution. Each experiment was accompanied by trans-stylbene to confirm the metabolic activity. The yeast two-hybrid cells were pre-incubated overnight at 30°C with vigorous shaking in a SD medium free from tryptophan and leucine, then diluted with 1.5 volumes of fresh 2 x SD medium. In a small test tube, 125 µL of the cell solution and 125 µL of the metabolite solution were mixed and then incubated at 30°C for 4 h. Thereafter, the same procedure as described in the first part was carried out.
- Data analysis
The results were evaluated on the basis of the relative activity, expressed as 10% relative effective concentration (REC10), which is the concentration of the test chemical showing 10% of the agonist activity of 10xE-6 mol/L 17-beta-Estradiol, the highest activity level of 17-beta-Estradiol. When the activity of the test chemical was higher than the REC10 within the concentration range tested, the chemical was judged to be positive. When it was judged to be negative, more than the highest dose tested was indicated.
Results and discussion
- Details on results:
- The test substance was negative in this test system. REC10 values of >1.0 x 10E-4 (parent compound) and >5.0 x 10E-5 (metabolite) were obtained.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.