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Short-term toxicity to fish

The lethal concentration (LC50) value of 5-nitrosalicylic acid in fish[Alburnus alburnus] in a 96 hr study on mortality effect was found to be 228.214 mg/L.Thus,considering the CLP Criteria for aquatic classification of the substance , it is concluded that 5-nitrosalicylic acid does not exhibit short term toxicity to fish [Alburnus alburnus].

Long-term toxicity to fish

According to annex IX of column I , Long-term toxicity testing on fish need not to be conducted as it is already provided as part of Annex VIII requirements.Therefore this study was considered to be waived.

Short-term toxicity to aquatic invertebrates

The effective concentration (EC50) value of 5-nitrosalicylic acid in aquatic invertebrate (Daphnia Magna) in a 48 hr study on the basis of immobilization effect was estimated to be 244.4349 mg/L. Thus considering the value from CLP Criteria for aquatic classification of the substance, it is concluded that 5-nitrosalicylic acid does not exhibits short term toxicity to aquatic invertebrate (Daphnia Magna).

Long-term toxicity to aquatic invertebrates

According to annex IX of column I ; Long-term toxicity testing on invertebrate (species : Daphnia) need not to be conducted as it is already provided as a part of Annex VII requirements.Therefore this study was considered to be waived.

Toxicity to aquatic algae and cyanobacteria

The no observed effect concentration (NOEC) of 5-nitrosalicylic acid in a aquatic green algae (Pseudokirchnerella subcapitata) in a 72 hr study on the basis of growth effect was estimated to be 5.3331mg/L.

Toxicity to microorganisms

The inhibition growth concentration (IGC50) value of 5-nitrosalicylic acid in microorganism (Tetrahymena pyriformis) in a 48 hr study on the basis of growth inhibition effect was estimated to be 127.266 mg/L.