Registration Dossier
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EC number: 203-737-8 | CAS number: 110-12-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 6-hour exposure followed by 14-day observation period
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Study conducted prior to introduction of Good Laboratory Practices; actual test report not available for review but sufficient data were provided to determine classification. Study was conducted by an internal Eastman Kodak Company method, developed prior to established guidelines. The results of this study are valid for classification insofar as the conditions of exposure are at least as stringent as modern guidelines.
Data source
Reference
- Reference Type:
- other: Eastman Kodak Company Basic Toxicity Summary Report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Method is an in vivo study using groups of four male rats exposed (whole body) to an atmosphere containing the test material for a period of six hours. After termination of exposure, the animals were observed for mortality and adverse clinical signs for a period of 14 days. Body weights were measured prior to exposure and at termination of the 2-week observation period. No gross pathology was performed at study termination.
- GLP compliance:
- no
- Test type:
- other: Study conducted according to an internal Eastman Kodak Company laboratory method using a 6-hour inhalation exposure period.
- Limit test:
- no
Test material
- Reference substance name:
- 5-methyl-2-hexanone
- IUPAC Name:
- 5-methyl-2-hexanone
- Reference substance name:
- 5-methylhexan-2-one
- EC Number:
- 203-737-8
- EC Name:
- 5-methylhexan-2-one
- Cas Number:
- 110-12-3
- Molecular formula:
- C7H14O
- IUPAC Name:
- 5-methylhexan-2-one
- Reference substance name:
- MIAK; Isoamyl methyl ketone
- IUPAC Name:
- MIAK; Isoamyl methyl ketone
- Details on test material:
- -Name of test material as cited in study report: Methyl isoamyl ketone
-Appearance: liquid
-Melting Point: -74 °C
-Boiling Point: 145 °C at 760 mm Hg
-Solubility: 0.5% in water at 20 °C
-Purity: 98%
-Impurities: 2% MIBK, 10-20 ppm HOMME
-Shipped to testing laboratory: May 16, 1978
-Source of test material: Tennesse Eastman Company (Eastman Chemical Company)
-Storage conditions: room temperature and humidity
Constituent 1
Constituent 2
Constituent 3
Test animals
- Species:
- rat
- Strain:
- not specified
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- Weight Range at Study Initiation: 181-233 grams
Administration / exposure
- Route of administration:
- inhalation
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: air
- Details on inhalation exposure:
- Rats were exposed (whole-body) to target vapor concentrations of 800, 1600, 3200, or 6400 ppm of the test material for 6 hours. The test material was vaporized, diluted with ambient (room temperature) air, then directed to a 20-liter glass bell jar for exposure, and monitored by infrared spectroscopy. Actual mean vapor concentrations achieved were 802, 1603, 3207, and 5878 ppm.
- Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- Atmosphere concentrations monitored by infrared spectroscopy.
- Duration of exposure:
- 6 h
- Concentrations:
- Mean vapor concentrations achieved were 802, 1603, 3207, and 5878 ppm.
- No. of animals per sex per dose:
- 4 males/dose concentration
- Control animals:
- not specified
- Details on study design:
- Groups of four male rats (strain not available) with a weight range of 181-233 grams were exposed (whole-body) to target vapor concentrations of 800, 1600, 3200, or 6400 ppm of the test material for 6 hours. The test material was vaporized, diluted with ambient (room temperature) air, then directed to a 20-liter glass bell jar for exposure, and monitored by infrared spectroscopy. Actual mean vapor concentrations achieved were 802, 1603, 3207, and 5878 ppm. During and subsequent to exposure, the animals were observed for mortality and adverse clinical signs for a period of 14 days. Body weights were measured prior to exposure and at termination of the 2-week observation period. No gross pathology was performed at study termination.
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- > 3 207 - < 5 875 ppm
- 95% CL:
- > 1 903 - < 3 805
- Exp. duration:
- 6 h
- Remarks on result:
- other: Based on mortality, the calculated LC50 (6-hr) was approximately 3813 ppm (17806 mg/m3).
- Mortality:
- All rats in the 5878 ppm concentration group died within 2.5 hours after the start of exposure. One of four rats in the 3207 ppm concentration group died just prior to termination of the 6-hour exposure.
- Clinical signs:
- other: At the 5878 ppm concentration level, eye irritation and narcosis were observed for all animals prior to death during the exposure period. Within 2 hours of the start of exposure, all animals exposed to a concentration of 3207 ppm displayed signs of eye ir
- Body weight:
- Although the three surviving rats in the 3207 ppm group lost weight (3-13 grams) between Day 0 and Day 3, subsequent weight gain was normal. All other rats gained weight normally over the 14-day study.
- Gross pathology:
- Not performed
Any other information on results incl. tables
An LC50 (6-hr) of 3813 ppm is equivalent to an LC50 (4-hr) of approximately 5000 ppm.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Remarks:
- Migrated information
- Conclusions:
- Under conditions used in this study, methyl isoamyl ketone was lethal by the inhalation route to all male rats exposed to a concentration of 5878 ppm for 2.5 hours. Since only one of four male rats died at a concentration of 3207 ppm, the LC50 (6-hr) in male rats was >3207 ppm but <5878 ppm. The calculated LC50 (6-hr) was approximately 3813 ppm which is equivalent to an LC50 (4-hr) of approximately 5000 ppm.
Based on a calculated inhalation LC50 (6-hr) value of 3813 ppm (equivalent to a 4-hr LC50 of approximately 5000 ppm), methyl isoamyl ketone is classified as Category IV for classification and labeling for acute lethality by the inhalation route under GHS.
Methyl isoamyl ketone currently carries an R20 Risk Phrase (Harmful if Inhaled) under Annex 1 of the European Union Dangerous Substances Directive. - Executive summary:
In an acute inhalation toxicity study, groups of four male rats were exposed (whole body) to methyl isoamyl ketone at vapor concentrations of 802, 1603, 3207, and 5878 ppm for a period of 6 hours. All rats in the 5878 ppm group and one of four rats in the 3207 ppm group died during the exposure period. At the 5878 ppm concentration level, eye irritation and narcosis were noted for all animals prior to death. Within 2 hours of exposure, all animals exposed to a concentration of 3207 ppm displayed signs of eye irritation, unresponsiveness, and impaired gait. By 4-5 hours of exposure, these rats were narcotized with depressed respiration. The three remaining rats at this concentration level recovered fully following cessation of exposure. At the 1603 ppm concentration level, abnormal signs were limited to sluggish responses after 6 hours of exposure. In the 802 ppm group, animals appeared alert during exposure, though immediately after exposure, animals appeared a bit sluggish. Although the three surviving rats in the 3207 ppm group lost weight (3-13 grams) between Day 0 and Day 3, subsequent weight gain was normal. All other rats gained weight normally over the 14-day study. The LC50 was calculated to be 3813 ppm for a 6-hour exposure. Methyl isoamyl ketone was toxic to rats at high airborne concentrations and caused reversible effects on the central nervous system.
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