Registration Dossier

Administrative data

Description of key information

Key value for chemical safety assessment

Skin sensitisation

Endpoint conclusion
Endpoint conclusion:
adverse effect observed (sensitising)
Additional information:

The potential of HC Blue no 2 to induce contact hypersensitivity was examined using the local lymph node assay (OECD guideline 429). Groups of 4 female mice in each were treated with 0.5%, 1%, 2.5%, 5% and 10% of the test substance in vehicle (dimethylformamide) for 3 daily treatments. After 2 days of resting, the proliferation of lymphocytes in the lymph node draining the application site was measured by incorporation of tritiated methyl thymidine (day 6). While the data is equivocal, the test substance HC Blue No 2 is considered to have a skin sensitising potential.


Migrated from Short description of key information:
The potential of HC Blue no 2 to induce contact hypersensitivity was examined using the local lymph node assay (OECD guideline 429). Groups of 4 female mice in each were treated with 0.5%, 1%, 2.5%, 5% and 10% of the test substance in vehicle (dimethylformamide) for 3 daily treatments. After 2 days of resting, the proliferation of lymphocytes in the lymph node draining the application site was measured by incorporation of tritiated methyl thymidine (day 6). While the data is equivocal, the test substance HC Blue No 2 is considered to have a skin sensitising potential.

Justification for classification or non-classification