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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The 96 hour LC50 for the test material HC Blue No 2 to rainbow trout (Oncorhynchus mykiss), based on the nominal test concentration was greater than 100 mg/L and correspondingly the No Observed Effect Concentration was greater than or equal to 100 mg/L. 

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

A study was performed to assess the acute toxicity of the test material, HC Blue No 2 to rainbow trout (Oncorhynchus mykiss). The method followed that decribed in the OECD guidelines for Testing of Chemicals (1992) No 203, ''Fish, Acute Toxicity Test' referenced as Method C.1 of Commission directive 92/69/EEC (which constitutes Annex V of Council Directive 67/548/EEC). Following apreliminary range-finding study fish were exposed, in two groups of ten, to an aqueous solution of the test material, at a single concentration of 100 mg/L for a period of 96 hours under semi-static test conditions. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the study until termination after 96 hours. The 96 hour LC50 for the test material HC Blue No 2 to rainbow trout (Oncorhynchus mykiss), based on the nominal test concentration was greater than 100 mg/L and correspondingly the No Observed Effect Concentration was greater than or equal to 100 mg/L.