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EC number: 700-916-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- This study is classified as reliable with restrictions because it is an acceptable, well-documented study report. Read-across justification: Based on the chemical composition, the renewable hydrocarbons produced from raw materials such as fatty acid rich oil like Crude Tall Oil (CTO) or triglyserides, using a hydrotreatment process have similar hydrocarbon fractions and they contain the same critical constituents than fossil diesel fuels. According to the identified hydrocarbon blocks, the typical carbon number ranges and the physicochemical properties, the renewable hydrocarbons with diesel type fractions can be considered as having structural similarities and similar behaviour in contact with water and in the physiological processes than the analogue source substances (fossil diesel fuels). Their irritation properties, skin sensitisation property as well as acute and long-term adverse effects to human health is similar. Therefore, and in order to avoid the unnecessary animal testing, the read-across data from the analogue fossil diesel fuels is used to evaluate skin and eye irritation, the genetic toxicity, carcinogenicity, developmental toxicity and short term and/or long-term toxicological effects of the target substance.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- GLP compliance:
- not specified
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- Distillates (petroleum), hydrodesulfurized middle
- IUPAC Name:
- Distillates (petroleum), hydrodesulfurized middle
- Reference substance name:
- Distillates (petroleum), hydrodesulfurized middle
- EC Number:
- 265-183-3
- EC Name:
- Distillates (petroleum), hydrodesulfurized middle
- IUPAC Name:
- 265-183-3
- Reference substance name:
- 64742-80-9
- Cas Number:
- 64742-80-9
- IUPAC Name:
- 64742-80-9
- Details on test material:
- - Name of test material (as cited in study report): API 81-10
- Substance type: Hydrodesulfurized Middle Distillate
- Physical state: Clear yellow liquid
Constituent 1
Constituent 2
Constituent 3
Results and discussion
Test results
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Remarks:
- weak
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Read-across justification with data matrices is presented in IUCLID section 13 (Annex I of CSR).
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive weakly positive
The study was judged by the authors as producing weak positive findings both in the presence or absence of S9. In view of the positive findings both in the presence and absence of S9, the poor repeatability and the absence of a dose response relationship, this study must be considered unreliable. - Executive summary:
Compositional and physico-chemical data show that Other Gas Oils are very similar to renewable hydrocarbons of wood origin (diesel type fraction). It is considered appropriate, therefore, to read across from the Other Gas Oil data to renewable hydrocarbons of wood origin (diesel type fraction).
In a mouse lymphoma mutagenicity assay, mouse lymphoma cell line, L5178Y, was exposed to the test material, hydrodesulfurised middle distillate in ethanol, in the presence and absence of rat liver S-9 for four hours. In the inactive system (no S-9), the cells were exposed to test material at concentrations ranging from 25 nl/ml to 200 nl/ml and in the active system (with S-9) the cells were exposed to test material at concentrations ranging from 12.5nl/ml to 300 nl/ml. In addition to treated cells, a solvent control group and positive controls consisting of ethylmethane sulfonate for inactive studies (no S-9) and 3-methylcholanthrene (MCA) for active studies (with S-9) were also used to test the efficacy of the mutagenicity assay. This assay was performed in two sets to ensure reproduction of experimental results.
In cells treated with the test material in the absence of S-9, a wide range of toxicities was noted, including high toxicity at higher concentrations (value not specified). Additionally, a repeatable increase in mutant frequency was noted, but this frequency was not dose-dependent. In cells treated with the test material in the presence of S-9 activation, toxicity was again noted at various concentrations (values not specified) along with repeatable increases in mutant frequency. These mutant frequencies, however, were not dose-dependent. Based on these results, the study authors concluded that hydrodesulfurised middle distillate is weakly mutagenic in the mouse lymphoma assay, both in the presence and absence of S-9 activation.
This study received a Klimisch rating of "reliable without restriction" because it closely followed OECD Guideline 476 and appears to be GLP compliant.
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