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Toxicological information

Dermal absorption

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Administrative data

Endpoint:
dermal absorption in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1975
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Methods described only in summary; no specific data for DNCB, no data for individual samples

Data source

Reference
Reference Type:
publication
Title:
Percutaneous absorption: On the relevance of in vitro data
Author:
Franz, T. J.
Year:
1975
Bibliographic source:
J. Invest. Dermatology 64: 190-195 (1975)

Materials and methods

Test guideline
Qualifier:
no guideline required
Principles of method if other than guideline:
Absorption through human skin in vitro using 14C labelled material, determination of total absorption
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
no data
Radiolabelling:
yes
Remarks:
14 C

Test animals

Species:
human
Sex:
not specified

Administration / exposure

Type of coverage:
open
Vehicle:
acetone
Duration of exposure:
60 h
Doses:
no exact data; 4 - 40 µg per skin sample
No. of animals per group:
not applicable
Control animals:
no
Remarks:
not applicable

Results and discussion

Percutaneous absorption
Dose:
see above
Parameter:
percentage
Absorption:
ca. 53 %
Remarks on result:
other: no data
Remarks:
no exact dose reported
Conversion factor human vs. animal skin:
human skin used

Any other information on results incl. tables

results are reported only as summary, no individual data

Applicant's summary and conclusion

Conclusions:
DNCB is absorbed readily through human skin in vitro.