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EC number: 202-551-4 | CAS number: 97-00-7
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: made according to GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1-chloro-2,4-dinitrobenzene
- EC Number:
- 202-551-4
- EC Name:
- 1-chloro-2,4-dinitrobenzene
- Cas Number:
- 97-00-7
- Molecular formula:
- C6H3ClN2O4
- IUPAC Name:
- 1-chloro-2,4-dinitrobenzene
- Details on test material:
- - Appearance: yellow solidified melt
- Purity: 99.7 %
- Stability under test conditions: stable
Constituent 1
Test solutions
- Details on test solutions:
- Tests concentrations: 0.032, 0.056, 0.1, 0.18, 0.32, 0.56 and 1.0 mg/l
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Strain: 86.81 SAG
Study design
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 24 - 26 ºC
- Details on test conditions:
- - Medium: OECD algae medium
- Light intensity and quality: constantly illuminated with a light intensity of 150 μE.m-2.s-1 temperated water bath with constant motion of 100 cycles/min.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.392 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.151 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.067 mg/L
Applicant's summary and conclusion
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