Registration Dossier

Administrative data

Endpoint:
long-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: conforms to guideline & GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report Date:
1994

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Analytical purity: 99.7 %

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Substance concentration determined before study and once a week until the end

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:
flow-through
Water media type:
freshwater
Total exposure duration:
28 d

Test conditions

Hardness:
2.5 - 2.7 mmol/l
Test temperature:
21 - 23 ºC
pH:
7.9 - 8.3
Details on test conditions:
Conductivity: 630 - 700 uS/cm
Photoperiod: 6 am to 6 pm
Light intensity: approx. 700 lux

Test concentrations: 0 / 0.005 / 0.016 / 0.05 / 0.16 / 0.5 mg/l (10 fish used per concentration)

Results and discussion

Effect concentrationsopen allclose all
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
0.16 mg/L
Basis for effect:
mortality
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
0.05 mg/L
Basis for effect:
mortality
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
0.05 mg/L
Basis for effect:
other: any observed effect
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
0.016 mg/L
Basis for effect:
other: any observed effect
Details on results:
No lethality occurred in the concentration of 0.05 mg/l or less.
Results with reference substance (positive control):
No lethality occurred in the control group.

Applicant's summary and conclusion