Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
25.10.1994 till 01.12.1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline conform study under GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report Date:
1995

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
yes
Remarks:
The vessel size, the bacterial density and the concentration of test material differ
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
purity: 99,7 %
Stability in water solution: 6 weeks

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, domestic, adapted
Details on inoculum:
- pre-adaptation of inoculum from municipal wastewater treatment plant Frankfurt Sindlingen
- type of inoculum: activated sludge was a centrifuged sediment washed, again centrifuged and resuspended
- pre-adapted inoculum: 12 g/l of inoculum, based on wet mud, were preadapted in the presence of 25 mg/l of test substance over 29 days with stirring and airing.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
2.87 mg/L
Based on:
test mat.

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
< 20
Sampling time:
28 d

Applicant's summary and conclusion

Interpretation of results:
not inherently biodegradable