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EC number: 700-776-7 | CAS number: -
Ecotoxicological information
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study according to OECD 201
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of mono-[(1,3-dihydro-1,3-dioxo-2H-isoindol-2-yl)methyl]-5,12-dihydroquino[2,3-b]acridine-7,14-dione and di-[(1,3-dihydro-1,3-dioxo-2H-isoindol-2-yl)methyl]-5,12-dihydroquino[2,3-b]acridine-7,14-dione and 5,12-dihydroquino[2,3-b]acridine-7,14-dione
- EC Number:
- 700-776-7
- Molecular formula:
- C29H17O4N3 (unspecified isomers) / C38H22O6N4 (unspecified isomers) / C20H12O2N2
- IUPAC Name:
- Reaction mass of mono-[(1,3-dihydro-1,3-dioxo-2H-isoindol-2-yl)methyl]-5,12-dihydroquino[2,3-b]acridine-7,14-dione and di-[(1,3-dihydro-1,3-dioxo-2H-isoindol-2-yl)methyl]-5,12-dihydroquino[2,3-b]acridine-7,14-dione and 5,12-dihydroquino[2,3-b]acridine-7,14-dione
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Physical state: Red powder
- Stability under test conditions: Not specified, assumed to be stable.
- Storage condition of test material: Room temperature, protected from light, in original container.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The water accommodated fraction (WAF) was prepared with a nominal loading of the test item of 100 mg/L.
100 mg/L of the test item was weighed out and transferred with an appropriate amount of dilution water into a brown glass flask. This dispersion was shaken for 24 hours with 20 rpm at room temperature. After a separation phase of 1 hour, the water accommodated fraction was taken from the homogenous liquid phase. The aqueous phase or WAF was removed by siphoning (from the approximate centre of the glass flask) to give the 100 mg/L loading rate WAF to be used as a stock WAF.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: Desmodesmus subspicatus CHODAT SAG 86.81
- Source (laboratory, culture collection): Sammlung von Algenkulturen (SAG), Pflanzenphysiologisches lnstitut der Universitat Gottingen, Nikolausberger Weg 18, D-37073 Gottingen.
- Age of inoculum (at test initiation): A four day old preculture, prepared in dilution water, was used as inoculum.
- Method of cultivation: Fresh stocks are prepared every month on Z-Agar. Light intensity amounts to 35-70 µE · mE-2 * s^-1 for 24 h per day.
- Culture medium: Nutrient medium Z according to LOTTGE et al. (1994)
- Age of inoculum (at test initiation):
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21 - 24 °C, controlled at± 2 °C.
- pH:
- 7.99 to 8.40
- Nominal and measured concentrations:
- WAF: 100 mg/L (loading rate)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Sterile Erlenmeyer flasks (vol. 250 ml) with cotton wool plugs.
- Aeration: No
- Initial cells density:
Nominal: approximately 2- 5 x 10E3 cells/ml
Actual: 4.332 x 10E3 cells/ml
- No. of organisms per vessel:
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: yes/no
- Adjustment of pH:
- Photoperiod: 24 hours/day light
- Light intensity and quality: 60 - 120 µE * m^-2 * s^-1
- Light source: Fluorescent tubes, OS RAM L 36 W /11-865, cool daylight
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Cell density was measured daily via Chlorophyll-a- fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as background signal.
The pH-value at the beginning of the exposure was measured in one additional replicate of the limit loading and the control. At the end of the exposure, it was measured in a pooled sample of the limit loading and the control. The room temperature was measured continuously. Light intensity was measured prior to the start of the test.
Algae cells were evaluated microscopically at the start and the end of the incubation period. The cells were checked for unusual cell shapes, colour differences, differences in chloroplast morphology, flocculation, adherence of algae to test containers and agglutination of algal cells. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate
- Basis for effect:
- growth rate
- Remarks on result:
- other: No acute toxic effects at the range of solubility.
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate
- Basis for effect:
- growth rate
- Remarks on result:
- other: No acute toxic effects at the range of solubility.
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate
- Basis for effect:
- biomass
- Remarks on result:
- other: No acute toxic effects at the range of solubility.
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate
- Basis for effect:
- biomass
- Remarks on result:
- other: No acute toxic effects at the range of solubility.
- Results with reference substance (positive control):
- see "Any other information on results incl. tables"
Any other information on results incl. tables
EC50 Values of the Reference Item (based on nominal concentrations [mg/L], (0-72 hours))
| Actual Study | Valid Range (average ± 3 x SD) | |
Rate-related inhibition |
||
| ErC50 | 0.631 | 0.682 ± 0.452 |
| 95 % confidence interval | 0.583- 0.687 | |
Yield inhibition |
||
| EyC50 | 0.256 | 0. 314 ± 0.144 |
| 95 % confidence interval | 0.247- 0.265 | |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- No acute effects occur within the range of solubility. The test substance is with high probability acutely not harmful to aquatic algae.
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