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EC number: 272-712-1
CAS number: 68909-77-3
The residuum from the reaction of diethylene glycol and ammonia. It consists predominantly of morpholine-based derivatives such as [(aminoethoxy)ethyl]morpholine, [(hydroxyethoxy)ethyl]morpholine, 3-morpholinone, and 4,4'-(oxydi-2,1-ethanediyl)bis[morpholine].
A synthetic sewage of the following
composition, was added to each test vessel to act as a respiratory
dissolved in 1 litre of water with the aid of ultrasonication.
Table1 Oxygen Consumption
Rates and Percentage Inhibition Values after 3 Hours Contact Time
[Increase in respiration rate as
compared to controls]
Replicates 1 to 2
Table 2 pH Values of
the Test Preparations at the Start and End of the Exposure Period
Replicates 1 to 2
3 Observations on the Test Preparations Throughout
the Test Period
Observations on Test Preparations
Dark brown dispersion
Clear colourless solution, no undissolved test item visible*
Dark brown dispersion, no undissolved test item visible
Extremely pale brown solution, no undissolved test item visible *
Pale brown solution, no undissolved test item visible *
Dark brown dispersion, no undissolved reference item visible
Replicates 1 to 2
*Observations made prior
to the addition of synthetic sewage and activated sewage
A study was performed to assess the effect of the test item on the respiration of activated sewage sludge. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984) No 209 "Activated Sludge, Respiration Inhibition Test", Method C.11 of Commission Regulation (EC) No. 440/2008 and US EPA Draft Ecological Effects Test Guidelines OPPTS 850.6800.
Activated sewage sludge was exposed to an aqueous solution of the test item at concentrations of 10, 32, 100, 320 and 1000 mg/l for a period of 3 hours at a temperature of 21 ± 1°C with the addition of a synthetic sewage as a respiratory substrate.
The rate of respiration was determined after 3 hours contact and compared to data for the control and a reference item, 3,5-dichlorophenol.
The effect of the test item on the respiration of activated sewage sludge gave a 3-Hour EC50of greater than 1000 mg/l. The No Observed Effect Concentration (NOEC) after 3 hours exposure was1000mg/l.
It was considered unnecessary and unrealistic to test at concentrations in excess of 1000 mg/l.
The reference item gave a 3-Hour EC50value of 7.0 mg/l, 95% confidence limits 5.4 - 9.0 mg/l.
The effect of the test item on the respiration of activated sewage sludge micro-organisms gave a 3-Hour EC50of greater than 1000 mg/l. The No Observed Effect Concentration (NOEC) after 3 hours exposure was 1000 mg/l.
An activated sludge inhibition test (Clarke, 2010 - report 41004022) carried out according to the OECD guideline 209 with activated sludge resulted in a 3h-EC50 of >1000 mg/L and a 3h-NOEC of 1000 mg/L. The study is given a Klimisch score of 1, was conducted under GLP and is considered as the key study for endpoint coverage.
The study of Clarke (2010 - report 41004022) was performed to assess the effect of the test item on the respiration of activated sewage sludge. The method followed that described in the OECD guideline 209.
The effect of the test item on the respiration of activated sewage sludge gave a 3h-EC50 of greater than 1000 mg/l. The No Observed Effect Concentration (NOEC) after 3 hours exposure was 1000mg/l.
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