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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
other information
Study period:
2007-09-17 to 2007-10-15 (experimental phase)
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Batch-Identification: 76584075L0
- Purity: 99.8 area-%
- Homogeneity: Homogeneous
- Storage stability: Expiry date: 17 January 2009
- Physical state/ Appearance: Liquid / colorless, clear

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge: STP Mannheim, Baden Wuerttemberg, Germany
- Storage conditions: Aeration in the laboratory until use
- Pretreatment: A suitable aliquot of the activated sludge suspension was sieved by a finely woven mesh with a mesh size about 1 mm. After settling the supernatant was discarded and the sludge suspension was filled up with tap water. The washing step was repeated three times. Afterwards the sludge concentration of the suspension was adjusted to a concentration of 6.0 g/L dry weight and then added to the test vessels to obtain a sludge concentration of 30 mg/L dry substance.
- Concentration of sludge: 30 mg/L
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
20 mg/L
Based on:
DOC
Initial conc.:
29 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Composition of medium: According to guideline
- Test temperature: 22 ± 2°C
- Suspended solids concentration of the inoculum: 6 g/L

TEST SYSTEM
- Culturing apparatus: 2L conical flasks, filled up to a volume of 1000 mL
- Number of culture flasks/concentration: 2

SAMPLING
- Sampling frequency: on days 0, 1, 3, 5, 7, 10, 14, 17, 21, 24, 27 and 28

CONTROL AND BLANK SYSTEM
- Inoculum blank: 2
- Abiotic sterile control: 1
- Toxicity control: 1
- Reference substance: 1
- Adsorption control: 1

Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
< 10
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
Degradation of reference substance after 14 days: 96%

Any other information on results incl. tables

- Elimination of the test substance by adsorption (% DOC): < 10 after 5 days

- Physico-chemical (abiotic) elimination of the test substance (% DOC): < 10 at the end of exposure

- Biodegradation degree in the inhibition control afier 14 days (% DOC): 49

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed