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Diss Factsheets

Administrative data

Endpoint:
nanomaterial aspect ratio/shape
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2020
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference
Reference Type:
other: unpublished study report
Title:
Unnamed
Year:
2020
Report date:
2020

Materials and methods

Principles of method if other than guideline:
Method selection according to Gaillard et al., Nanoscale Adv., 2019, 1, 781
GLP compliance:
no
Type of method:
transmission electron microscopy
Details on methods and data evaluation:
TEM image evaluation according to Verleysen et al., Materials 2019, 12, 2274; doi:10.3390/ma12142274
Sample preparation: Dispersion in ethanol; transfer to carbon-coated TEM-grids.

Test material

Constituent 1
Chemical structure
Reference substance name:
2-cyano-2-[2,3-dihydro-3-(tetrahydro-2,4,6-trioxo-5(2H)-pyrimidinylidene)-1H-isoindol-1-ylidene]-N-methylacetamide
EC Number:
278-388-8
EC Name:
2-cyano-2-[2,3-dihydro-3-(tetrahydro-2,4,6-trioxo-5(2H)-pyrimidinylidene)-1H-isoindol-1-ylidene]-N-methylacetamide
Cas Number:
76199-85-4
Molecular formula:
C16H11N5O4
IUPAC Name:
2-cyano-N-methyl-2-[3-(2,4,6-trioxotetrahydropyrimidin-5(2H)-ylidene)-2,3-dihydro-1Hisoindol-1-ylidene]acetamide
Test material form:
solid: nanoform

Data gathering

Instruments:
TEM: Thermo-Fisher Talos 200 kV

Results and discussion

Shape description
Key result
Shape:
spherical
Remarks on result:
other: The material is assigned to the “spheroidal” shape category.
Aspect ratio
Key result
x Dimension:
2
St. dev.:
0.9
y Dimension:
1
Remarks on result:
other: median (D50)

Any other information on results incl. tables

The number-based concentration of particles with sizes below 100 nm (smallest feret diameter) is 98%. The median (D50) of the particle size is 48,4 nm.


The material is therefore classified as “nano” according to the definition of nanomaterial 2011/696/EU.

Applicant's summary and conclusion

Conclusions:
- The particles have a median aspect ratio of 2.0:1
- The shape is “spherical”. The material is assigned to the “spheroidal” shape category.
- The median (D50) of the particle size is 48.4 nm. The number-based concentration of particles below 100 nm (smallest feret diameter) is 98%.
The material is therefore classified as "nano" according to the definition of nanomaterial 2011/696/EU.