Reaction products of diazotized 2-amino-4-(methylsulfonyl)-phenol with 2-(3-Chlorophenyl)-2,4-dihydro-5-methyl-3H-pyrazol-3-one and Methyl-7-hydroxy-1-naphthylcarbamate – and chromium (III) 2:1, salted by ammonium, sodium and acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study according to OECD 203 including analytical monitoring and using an emulsifier

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Reaction products of diazotized 2-amino-4-(methylsulfonyl)-phenol with 2-(3-Chlorophenyl)-2,4-dihydro-5-methyl-3H-pyrazol-3-one and Methyl-7-hydroxy-1-naphthylcarbamate – and chromium (III) 2:1, salted by ammonium, sodium and acid

EC Number:

936-609-1

Molecular formula:

C40H32CrN4O12S2-R+] . [C36H28ClCrN7O10S2-R+] . [C34H26Cl2CrN8O8S2-R+]; R+ = NH4+, Na+ or H+

IUPAC Name:

Reaction products of diazotized 2-amino-4-(methylsulfonyl)-phenol with 2-(3-Chlorophenyl)-2,4-dihydro-5-methyl-3H-pyrazol-3-one and Methyl-7-hydroxy-1-naphthylcarbamate – and chromium (III) 2:1, salted by ammonium, sodium and acid

Details on test material:

- Physical state: brown powder
- Analytical purity:
- Storage condition of test material: room temperature

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Sampling method: Samples for analysis were taken at the start and at the end of the exposure.

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Stock solution: 2.50 g test item were dissolved in and made up to 25 ml with tetrahydrofuran (THF).
- Method: Calculated amounts of test substance to produce the desired test concentrations were given into the water and were homogeneously distributed.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): No. The test substance appeared homogeneously distributed in the test concentrations.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra-Fish
- Source: West Aquarium / D-3422 Bad Lauterberg
- Length at study initiation (length definition, mean, range and SD): 36 mm (23-28 mm)
- Weight at study initiation (mean and range, SD): 0.15 g (0.12-0.19 g)

ACCLIMATION
- Acclimation period: 33 days
- Adaption: 24 hours / no food 24 hours prior to exposure

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96

Remarks on exposure duration:

h

Test conditions

Hardness:

175 mg CaCO3/L

Test temperature:

23 ± 1°C

pH:

8.0 - 8.3

Dissolved oxygen:

84 - 97 %

Nominal and measured concentrations:

nominal: 1.0, 1.8, 3.2, 5.8, 10 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria of 20 liters filled with 15 liters (36/22/25 cm)
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.10 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water (carbon filter)
- Intervals of water quality measurement: oxygen, pH, temperature daily

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Fluorescent light, 16 hours daily

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 1.1-1.6 mg/L

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

1.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test item is acutely toxic to fish.