Registration Dossier

Toxicological information

Genetic toxicity: in vitro

Currently viewing:

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1984

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Lead acetate genotoxicity was tested at four laboratories in reverse mutation assays with S. typhimurium (TA98, TA100, TA1535, TA 1537, TA1538) and E. coli (WP-2 uvrA) at concentrations from 0.3 to 333.3 ug/plate. Tests were conducted with and without metabolic activation (S9 mix).
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent

Results and discussion

Test results
Species / strain:
other: S. typhimurium TA98, TA100, TA1535, TA1537, TA1538; E. coli WP2 uvrA
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity:
yes
Vehicle controls valid:
yes
Negative controls valid:
yes
Positive controls valid:
yes
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

Lead acetate was negative for genotoxicity in Salmonella and E. coli in all four laboratories tested.

Assay Method

Compound

Result

Dose or concentration

Reference

Salmonella typhimuriumTA100, TA1535, TA1537, TA1538, TA98, reverse mutation

Pb Acetate

-

333 μg/plate

Dunkel et al. (1984)

Escherichia coliWP-2 uvrA, reverse mutation

Pb Acetate

-

333 μg/plate

Dunkel et al. (1984)

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative
Executive summary:

Lead acetate genotoxicity was tested at four laboratories in reverse mutation assays with S. typhimurium (TA98, TA100, TA1535, TA 1537, TA1538) and E. coli (WP-2 uvrA) at concentrations from 0.3 to 333.3 ug/plate. Tests were conducted with and without metabolic activation (S9 mix). Lead acetate was negative for genotoxicity in Salmonella and E. coli in all four laboratories tested.