Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
19.06.2006 - 23.06.2006
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
other: OSPAR Commission 2005: Protocols on Methods for the Testing of Chemicals used in the Offshore Industry (reference number: 2005-11 [a revised version of agreement 1995-07])
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): fluorphenylepoxyethan
- Physical state: liquid

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: (Water accomodated fraction (WAF)): 40, 100, 200, 400 and 1000 ml WAF/l
- Sampling method: 2 x 50 ml of stock solution
- Sample storage conditions before analysis: Freezer at -20ºC

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Cyprinodon variegatus
Details on test organisms:
TEST ORGANISM
- Common name: Sheephead minnow
- Source: originally obtained from a stock culture at Opus Plus Ltd.(the former ERT Orkney water Technology Centre, and cultured at DHI since June 2003. Animals from this DHI culture was used for this study

ACCLIMATION
- Acclimation conditions : no acclimatation needed, test done under same conditions at same facility as test animals was optained from.

Study design

Test type:
semi-static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
21±0.5ºC
pH:
7.9-8.1
Dissolved oxygen:
> 70%
Salinity:
31 ‰
Nominal and measured concentrations:
Stock solution made by 1000 mg substance dissolved in 2000 ml test media.
Nominal concentration: 500 mg/l.
Substance was not fully dissolved, therfore a test was taken from the middle of the volumetric flask (water accomodated fraction = WAF)
The stock solution was analysed to be 16.63 mg/l.
Details on test conditions:
TEST SYSTEM
- Test vessel: 2 L aquariums containing 1 L test solution
- Renewal rate of test solution (frequency/flow rate): after 48 h.
- No. of organisms per vessel: 10
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol (3,5-CDP), CAS No. : 591-35-5, at 2.3 mg/L & 4.6 mg/l

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC10
Effect conc.:
3.33 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
4.26 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
mortality
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Mortality: 50% at 2.3 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
Mortality in controls < 10 %, oxygen > 60%, mortality with reference test within acceptable range, 20-80 %, average weight of the fish was 0.06±0.02 g giving maximal 1 gram fish per liter testsolution
Conclusions:
LC50(96 h) of fluorphenylepoxyethan, CAS No. 88374-05-4 is 4.26 mg/L for Cyprinodon variegatus