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In an acute toxicity test with Rainbow trout (reliability category 1) performed as a limit test at 100 mg/L (measured, flow through), no signes of toxicity were observed in accordance with the results of a range finding test: NOEC (96h) = 100 mg/L (measured), LC50 (96h) > 100 mg/L (measured). The study was based on the ISO International Standard 7346-3: Flow-through method, 1996. In addition, the procedures were designed to meet the test methods and validity criteria of the EEC directive 92/69, Part C.1, 1992 and the OECD guideline No. 203, 1992. The study was performed compliant to GLP.

In a fish embry toxicity test (reliability category 2) performed similar to an OECD draft guideline ("Fish Embryo Toxicity (FET) Test", May 30, 2006 (1st Version) proposed to constitute an alternative test method to the acute toxicity tests (OECD 203), the follwing effect concentration of the test item was determined:

LC50 (48h) = 115.3 mg/L (105.9-125.5).

As no pH adjustment had been performed, pH-related effect at this concentration cannot be excluded. In any case, observed LC50 (48h) is above 100 mg/L thus corroborating the results of toe OECD 203 test described above.

In an acute toxicity test with Daphnia magna (48 hours, flow through, analytical confirmation of test item concentrations) the following results were obtained:

NOEC (48 h): 32 mg/L

EC50 (48 h): >100 mg/L

20-25% immobilisation was observed at target concentrations of 56 and 100 mg/L.

The study procedures were based on the ISO International Standard 6341, 1996. In addition, the test methods and validity criteria of the EEC directive 92/69, Part C.2, 1992 and the OECD guideline No. 202, 2004 were met.

In a 72 hour inhibition test with freshwater algae (Selenastrum capricornutum), the test item reduced total cell growth and growth rate significantly at 22 mg/L and higher:

EC10 (72h, growth rate): 24 mg/L;

EC50 (72h, growth rate): 88 mg/L;

NOEC (72h, biomass and growth rate): 10 mg/L

The test was performed according to ISO International Standard 8692, 2004. In addition, the procedures were designed to meet the test methods and validity criteria of the EEC directive 92/69, Part C.3, 1992 and the OECD guideline No. 201, 1984. Test item concentrations were analytically verified and in agreement with nominal concentrations.