1,4-dimethylpiperazine

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

No data

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Standardised study to determine the acute toxicity of the test item in mice after single i.p. application.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,4-dimethylpiperazine

EC Number:

203-412-0

EC Name:

1,4-dimethylpiperazine

Cas Number:

106-58-1

Molecular formula:

C6H14N2

IUPAC Name:

1,4-dimethylpiperazine

Details on test material:

- Name of test material (as cited in study report): Jeffcat DMP

Specific details on test material used for the study:

- Name of test material (as cited in study report): N,N´-Dimethylpiperazin
- Analytical purity: 99 %

Test animals

Species:

mouse

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 19-36 g
No further data.

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

water

Details on exposure:

No data

Doses:

200, 400, 640, 800 cm³/kg bw (170, 340, 544, 680 mg/kg bw - conversation in mg/kg is based on the density: d= 0.85 g/cm³)
The doses were administered as aqueous solutions of 8% (800 cm³/kg), 4% (400, 640 cm³/kg) and 2% (200 cm³/kg) test substance.

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations: daily
- Frequency of weighing: before the start of the study
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 544 - < 680 mg/kg bw

Based on:

test mat.

Remarks on result:

other: Corresponds to 640 and 800 cm³/kg bw; the mg/kg was calculated on the density d= 0.85 g/cm³. 680 mg/kg: all females and 4 males died within 24 hours post application; 544 mg/kg bw: one male and one female animal died within 24 hours post application.

Mortality:

680 mg/kg bw : all female animals and 4 males died within 24 hours post application
544 mg/kg bw : 1 male and 1 female animal died within 24 hours post application
340 mg/kg bw: one female animal died within 7 days post application
No mortalities in the 170 mg/kg bw dose group.

Clinical signs:

Immediately after application, dyspnoea, tremor, indicated morphine tails, shrunken flanks, high stepping gait, struggles in the 680 mg/kg bw dose group were observed. On the next day, the surviving animals showed clotted eyes, scrubby fur, accelerated respiration. 5 to 6 days after exposure, no clinical signs were observed.

Body weight:

no data

Gross pathology:

Animals that died: livers were full of blood, intestine and intraperitoneum were reddened
Animals sacrificed at the end of observation period: connation of liver and stomach in 10 animals

Applicant's summary and conclusion

Conclusions:

After intraperitoneal administration of the substance to mice, the LD50 was observed to be within the range of 544 and 680 mg/kg bw.