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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
No data
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1968
Report date:
1968

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Version / remarks:
(adopted on 1981, 12th may; inhalation hazard test)
Deviations:
yes
Remarks:
Animals were observed for only 7 days, no details only animal husbandry, exposure period of 8 hours, concentration of test substance in air mixture was not determined analytically
GLP compliance:
no
Test type:
other: inhalation hazard test (IHT)

Test material

Constituent 1
Chemical structure
Reference substance name:
1,4-dimethylpiperazine
EC Number:
203-412-0
EC Name:
1,4-dimethylpiperazine
Cas Number:
106-58-1
Molecular formula:
C6H14N2
IUPAC Name:
1,4-dimethylpiperazine
Details on test material:
- Name of test material (as cited in study report): Jeffcat DMP
Specific details on test material used for the study:
- Name of test material (as cited in study report): N,N´-Dimethylpiperazin
- Analytical purity: 99 %

Test animals

Species:
rat
Strain:
not specified
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 177 g (mean)

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
not specified
Vehicle:
air
Details on inhalation exposure:
The test demonstrates the toxicity of an atmosphere saturated with vapours of the volatile components of a test substance at the temperature chosen for vapour generation (20 °C). 3 rats (respec. 6 rats in the 8h experiment) per sex were exposed sequentially to the vapours, generated by bubbling 200 l/h air through a substance column of about 5 cm above a fritted glassdisc in a glass cylinder for 1 h, 3 h or 8 h. The documentation of clinical signs was performed over a period of 8 days.
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
>= 1 - <= 8 h
Remarks on duration:
1 h, 3 h or 8 h exposure
Concentrations:
1 h and 3 h exposure: 17.2 mg/L (mean); no data concerning 8 h exposure.
(nominal concentrations not verified by analytics).
No. of animals per sex per dose:
1 h and 3 h exposure: 6 animals
8 h exposure: 12 animals
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 7 days
- Frequency of observations: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
other: Inhalation Risk Test
Based on:
test mat.
Remarks on result:
other: exposure of 1, 3 or 8 hours (3 h exposure: 1 rat died within 24 hours; 8 h exposure: 1 animal died within the exposure, 9 animals within 24 h and 1 animal within 72 h after exposure)
Mortality:
1 h exposure: no mortality observed.
3 h exposure: 1 rat died within 24 hours.
8 h exposure: 1 animal died within the exposure, 9 animals within 24 h and 1 animal within 72 h after exposure.
Clinical signs:
other:
Body weight:
The surviving animals gained weight.
Gross pathology:
1 h exposure: no abnormalities observed.
3 h exposure: 1 animal showed ruffled fur and slight bloody crusts at the muzzle and orbital cavity. 1 animal had pulmonary emphysema with bronchitis.
8 h exposure: animals showed ruffled fur and bloody crust formation at the nose (4x), muzzles and orbital cavity (1x). 2 animals had pulmonary emphysema.

Any other information on results incl. tables

Mortality:

 24 h  7 days
 1 h exposure  0/6  0/6
 3 h exposure  1/6  1/6
 8 h exposure  10/12  11/12

The inhalation of a enriched/saturated vapor-air-mixture caused mortality within 3 h. There is indication that the test substance causes local irritation to exposed tissue including respiratory tract.

Applicant's summary and conclusion

Interpretation of results:
study cannot be used for classification
Conclusions:
The inhalation of a enriched/saturated vapor-air-mixture caused mortality within 3 h. There is indication that the test substance causes local irritation to exposed tissue including respiratory tract.