Cyclododecanone, 2-(2-methyl-2-propen-1-yl)-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study without restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-(2-methylprop-2-en-1-yl)cyclododecan-1-one

EC Number:

691-657-8

Cas Number:

62599-49-9

Molecular formula:

C16 H28 O

IUPAC Name:

2-(2-methylprop-2-en-1-yl)cyclododecan-1-one

Details on test material:

- Name of test material (as cited in study report): 2-(2-Methylallyl)-cyclododecanon-Rein
- Physical state: liquid / colorless, clear
- Analytical purity: 94-99%
- Lot/batch No.: (MACD Probe aus B410.16 vom 04.10.2012) xxxxxxxxxxx
- Storage condition of test material: Storage at room temperature

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal activated sludge from the wastewater treatment plant of Mannheim, Germany
- Storage conditions: The inoculum was collected from the aeration tank of the plant and aerated in the laboratory until use. A suitable aliquot of the activated sludge suspension was sieved by a finely woven mesh with a mesh size about 1 mm. To reduce the content of inorganic carbon in the blank controls the activated sludge suspension was pre-aerated for about 24 hours with synthetic air at a temperature of 22 ± 2 °C.
- Preparation of inoculum for exposure: At the day of exposure the suspension was washed one time with tap water. Therefore the aeration was stopped and the sludge was allowed to settle. After settling the supernatant was discarded and the remaining sludge suspension was filled up with tap water and the concentration of the sludge was adjusted to 6.0 g/L dry weight.

- Pretreatment: none
- Concentration of sludge:
Aliquots of 7.5 mL was added to the test vessels to obtain a sludge concentration of 30
mg/L dry weight.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

25 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

20 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: according to guideline
- Solubilising agent (type and concentration if used): no
- Test temperature: 22 ± 2 °C
- pH: The pH-values in the test vessels were measured and adjusted to 7.4 ± 0.2, if necessary



TEST SYSTEM
- Culturing apparatus: 2 L incubation bottles
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: pre-aeration for two days before test start
- Measuring equipment: carbon dioxide trap
- Details of trap for CO2 and volatile organics if used: Usually twice a week the Total Inorganic
Carbon (TIC) values of the adsorption solutions of the first trap were determined and
used for the calculation of the produced carbon dioxide. After each sampling the second
trap was moved forward and the new trap with fresh sodium hydroxide solution was
placed into the second position. Each trap was analyzed separately
- Test performed in open system: no


SAMPLING
- Sampling frequency: Usually twice a week
Samples for the TIC- and DOC-analysis were performed as repeat determination, using a
TOC-analyzer equipped with an auto sampler (Shimadzu TOC-5000A). The system works
with a combustion/non-disperse infrared gas analysis method.

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Inhibition control: yes

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

< 10

Sampling time:

28 d

Details on results:

2-(2-Methylallyl)-cyclododecanon-Rein was not readily biodegradable under the conditions of this test.

BOD5 / COD results

Results with reference substance:

Degree of biodegradation of the reference substance after 14 days: 69 % CO2/ThCO2