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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable publication which meets basic scientific principles

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Salmonella mutagenicity tests. III. Results from the testing of 225 chemicals
Author:
Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K and Speck W
Year:
1987
Bibliographic source:
Environ. Mutagen. Vol. 9 (Suppl 9) 1-109
Reference Type:
other: database
Title:
National Toxicology Progam - Database Search Application
Author:
NTP database
Year:
2011
Bibliographic source:
http://ntp-apps.niehs.nih.gov/ntp_tox/index.cfm

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
3,3'-iminodi(propylamine)
EC Number:
200-261-2
EC Name:
3,3'-iminodi(propylamine)
Cas Number:
56-18-8
Molecular formula:
C6H17N3
IUPAC Name:
bis(3-aminopropyl)amine

Method

Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Metabolic activation system:
liver S-9 from Aroclor-induced male Sprague-Dawley rats and Syrian hamsters
Test concentrations with justification for top dose:
0, 33, 100, 333, 1000, 1666, 3333 and 10000 µg/plate (preincubation test)
Vehicle / solvent:
- Vehicle used: water
Controls
Untreated negative controls:
yes
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
not specified
Details on test system and experimental conditions:
NUMBER OF REPLICATIONS: 2

Results and discussion

Test results
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
valid
Positive controls validity:
valid
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'. Remarks: S. typhimurium TA 98, TA 100, TA 1535, TA 1537

Applicant's summary and conclusion

Conclusions:
Dipropylenetriamine did not cause gene mutations in Salmonella typhimurium (Ames test).