2,5,8,11-tetramethyldodec-6-yne-5,8-diol

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1979

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

One group of ten (5 male and 5 female) albino rats was used in this study. The rats were placed in a 70 liter, all glass exposure chamber and exposed to a saturated atmosphere of the test material in air for one hour. The material was administered as an aerosol. the material was heated gently to approximately 135°F (57°C) to facilitate aerosolizing. The rate of flow was 10.0 liters per minute at a temperature of 70°F (21°C). The air was passed threough a desicant prior to being passed through the test material. By differential weighing it was calculated that the rats were subject to concentration of 20.1 mg/liter during the exposure period. This is an average value over the one hour period.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,5,8,11-tetramethyldodec-6-yne-5,8-diol

EC Number:

269-348-0

EC Name:

2,5,8,11-tetramethyldodec-6-yne-5,8-diol

Cas Number:

68227-33-8

Molecular formula:

C16H30O2

IUPAC Name:

2,5,8,11-tetramethyldodec-6-yne-5,8-diol

Constituent 2

Reference substance name:

2,5,8,11-tetramethyl-6-dodecyne-5,7-diol

IUPAC Name:

2,5,8,11-tetramethyl-6-dodecyne-5,7-diol

Test material form:

other: whithe solid waxy

Test animals

Species:

rat

Strain:

other: albino

Sex:

male/female

Details on test animals or test system and environmental conditions:

5 male & 5 female albino rats

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Chamber
- Exposure chamber volume: 70 liter

Analytical verification of test atmosphere concentrations:

yes

Remarks:

differential weighing

Duration of exposure:

14 d

Concentrations:

20.1 mg/liter

No. of animals per sex per dose:

5 male and 5 female

Control animals:

no

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 20.1 mg/L air (nominal)

Mortality:

none

Clinical signs:

other: Immediately after exposure the animals were ruffled, wheezing and dirty. Within 24 hours they appeared recovered and normal.

Body weight:

Average initial weight: 210 gr males and 205 gr females
Average final weight: 255 gr males and 230 gr females

Gross pathology:

Gross pathologic examination revealed nothing remarkable.

Any other information on results incl. tables

total dead 14 days:       0

total survived 14 days: 10 (all)

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

From the results obtained in this study using the method described above, it appears that the subject material has an LC50 greater than 20.1 mg/liter, the maximum concentration which could attained.