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EC number: 271-178-7 | CAS number: 68516-75-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-02-20 to 2012-02-21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: No vehicle, directly weighing, overnight stirring
- Eluate: Synthetic waste water acc. to OECD 209
- Differential loading: 1000 mg/L (actual limit concentration in the final test medium)
- Controls: Test medium without test or reference substance. 6 replicates: Triplicates at the beginning of the test + triplicates at the end of the test - Test organisms (species):
- activated sludge
- Details on inoculum:
- - Preparation of inoculum for exposure: Non adapted activated sludge from the municipal sewage treatment plant at Hildesheim D-31137 is well suited as it receives predominantly municipal sewage and hardly any industrial chemical waste.
- Pretreatment: washed twice and adjusted with chlorine free tap water in order to achieve a dry sludge concentration of 3.0 g/L ± 10 %.
The sludge was used within 24 h after sampling.
- Dry sludge concentration: 2.68 g/L (corresponding to 1.34 g/L suspended solids in the test vessel) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Hardness:
- Not determined
- Test temperature:
- nominal 18 - 22 °C, actual measured 20 - 22 °C
- pH:
- 7.40 (activated sludge)
7.50 (synthetic waste water) - Salinity:
- None, freshwater
- Nominal and measured concentrations:
- Test concentrations nominal: control and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 mL Erlenmeyer flask, DIN 12380
- Aeration: Permanent
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Composition of test medium:
250 mL inoculum
16 mL synthetic waste water
234 mL autoclaved tap water
Test or reference item concentration (directly weighed into each test vessel)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
After 3 h incubation, the oxygen concentration of each control. reference item and test item replicate was determined by means of oximeter immediately after transferring the solution to the measuring cell and the oxygen depletion was recorded for at least 3 minutes on a flat bed recorder.
TEST CONCENTRATIONS
- Range finding study
Inhibition of Respiration (Preliminary Tests)
Nominal Test Item Concentration Pretreatment Inhibition [%]
[mg/L]
10 Stirring overnight 4
100 -16
1000 9
1000 -1
1000 -4 - Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate p.a.
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50: 107 mg/L - Reported statistics and error estimates:
- The NOEC was determined by calculation of statistical significance of the inhibition of respiration in comparison to the control. One Way Analysis of Variance (ANOVA) was used for NOEC calculations. Before running the One Way Analysis of Variance a Normality test and an Equal Variance test were done first. P-values for both Normality and Equal Variance test were 0.05. The a-value (acceptable probability of incorrectly concluding that there is a difference) is a=0.05.
The EC-value of the reference item was calculated by by linear regression using software GraphPad Prism5, GRAPHPAD SOFTWARE, INC. Calculation of the confidence intervals for the EC50-value was carried out using standard procedures.
The coefficient of variation of the oxygen uptake rates of the control replicates was calculated with standard methods using Excel, MICROSOFT CORPORATION. - Validity criteria fulfilled:
- yes
- Conclusions:
- The inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriate low concentrations.
Reference
EC-Values with Confidence Interval of the test item
EC-values |
Confidence interval |
|
[mg/L] |
P = 95 [%] [mg/L] |
|
NOEC |
1000 |
- |
EC20 |
> 1000 |
- |
EC50 |
> 1000 |
n.d. |
EC80 |
> 1000 |
- |
Oxygen Uptake Rates, Specific
Respiration Rates and Inhibition of the Respiration
of Control, Reference and Test Item Concentrations
Reference and Test Item Concentration |
|
Oxygen Uptake Rate |
Specific Respiration Rate |
|
Mean Inhibition |
Control |
1 |
26.8 |
10.0 |
- |
- |
2 |
29.2 |
10.9 |
|||
3 |
29.6 |
11.0 |
|||
4 |
28.0 |
10.4 |
|||
5 |
28.0 |
10.4 |
|||
6 |
28.8 |
10.7 |
|||
58 |
1 |
22.4 |
21 |
22 |
|
2 |
22.0 |
23 |
|||
3 |
22.0 |
23 |
|||
100 |
1 |
15.2 |
46 |
46 |
|
2 |
14.8 |
48 |
|||
3 |
15.6 |
45 |
|||
180 |
1 |
7.6 |
73 |
74 |
|
2 |
7.6 |
73 |
|||
3 |
7.2 |
75 |
|||
1000 |
1 |
32.0 |
-13 |
-15 |
|
2 |
33.2 |
-17 |
|||
3 |
32.8 |
-15 |
Raw Data and Oxygen Uptake Rates of Test Item. Control and Reference Item
Test Solution / Concentration [mg/L] |
Repl. |
Oxygen Concentration* |
Measured distance** |
Oxygen uptake |
Control |
1 |
8.52 |
67 |
26.8 |
2 |
7.85 |
73 |
29.2 |
|
3 |
7.04 |
74 |
29.6 |
|
4 |
8.30 |
70 |
28.0 |
|
5 |
8.22 |
70 |
28.0 |
|
6 |
7.95 |
72 |
28.8 |
|
Reference Item 58 |
1 |
7.39 |
56 |
22.4 |
2 |
7.62 |
55 |
22.0 |
|
3 |
7.16 |
55 |
22.0 |
|
100 |
1 |
7.65 |
38 |
15.2 |
2 |
7.40 |
37 |
14.8 |
|
3 |
8.24 |
39 |
15.6 |
|
180 |
1 |
8.90 |
19 |
7.6 |
2 |
9.16 |
19 |
7.6 |
|
3 |
8.93 |
18 |
7.2 |
|
Test Item 1000 |
1 |
7.44 |
80 |
32.0 |
2 |
6.94 |
83 |
33.2 |
|
3 |
7.04 |
82 |
32.8 |
Description of key information
The inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriate low concentrations.
NOEC (3h) >= 1000 mg/L (nominal) for activated sludge (OECD 209)
Key value for chemical safety assessment
Additional information
For two ‘red disazo condensation pigments’(CAS 68516-75-6 and CAS 71566-54-6) the influence on the respiration of microorganisms were investigated in GLP guideline studies according to OECD 209 [DR.U.NOACK-LABORATORIEN, 2012; Experimental Toxicology and Ecology, BASF SE, 2012]. Both studies conducted with activated sludge from municipal sewage treatment plants, which predominantly receives domestic sewage. Up to the highest test concentration of 1000 mg/L (nominal) no inhibition on respiration observed after 3 hours of exposure.
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