Registration Dossier

Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study. The concentrations in the air are nominal concentrations and were not measured by a specific analysis. From the data given in the report the nominal concentrations cannot be calculated (data on flow rate, volume missing).

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report date:
1983

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Version / remarks:
1981
GLP compliance:
no
Test type:
standard acute method

Test material

Constituent 1
Chemical structure
Reference substance name:
1-cyanoallyl acetate
EC Number:
239-743-2
EC Name:
1-cyanoallyl acetate
Cas Number:
15667-63-7
Molecular formula:
C6H7NO2
IUPAC Name:
1-cyanoprop-2-en-1-yl acetate

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Kleintierfarm Madoerin AG, Fuellinsdorf/Switzerland
- Housing: except for exposure housing 5 per groups
- Diet: ad libitum:
- Water: ad libitum
- Acclimation period: one week prior to exposure


ENVIRONMENTAL CONDITIONS
according to guideline


Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
nose only
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Born & Co, Mechanische Werkstatt Moehlin/Switzerland
- Method of holding animals in test chamber: separate radial polyvinylchloride tubes
- Source and rate of air: no data, different air rates to get different concentrations
- Temperature in the air chamber: 20°C


TEST ATMOSPHERE
- Brief description of analytical method used: no specific analytical method, weighing of the glass bottle filled with test substance before starting the test and afterwards; from the weight difference and the known air volume the test article concentration [mg/L] was calculated
- Samples taken from breathing zone: no


Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
4 h
Concentrations:
6.5 mg/L
7.8 mg/L
9.3 mg/L
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observation hourly, after 24 h daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, necropsy
Statistics:
LOGIT estimation

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
LC50
Effect level:
7.9 mg/L air (nominal)
95% CL:
7.4 - 8.4
Exp. duration:
4 h
Sex:
male
Dose descriptor:
LC50
Effect level:
7.7 mg/L air (nominal)
95% CL:
6.7 - 8.5
Exp. duration:
4 h
Sex:
female
Dose descriptor:
LC50
Effect level:
8 mg/L air (nominal)
95% CL:
7.3 - 9.5
Exp. duration:
4 h
Mortality:
All deaths occured on the test day or killed in moribund state, respectively.
Clinical signs:
other: all dose groups: sedation, dyspnea, ruffled fur, rhinorrhea, ventral- and curved body position symptoms increased with increasing concentration
Body weight:
loss of body weight during observation period
Gross pathology:
lung: dark red discoloured and foam excretion, intestinal tract: meteorism

Applicant's summary and conclusion

Interpretation of results:
Category 3 based on GHS criteria
Remarks:
Migrated information