Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008
Report date:
2009

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Version / remarks:
2006
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
1-cyanoallyl acetate
EC Number:
239-743-2
EC Name:
1-cyanoallyl acetate
Cas Number:
15667-63-7
Molecular formula:
C6H7NO2
IUPAC Name:
1-cyanoprop-2-en-1-yl acetate

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Concentration of the test substance was determined in all concentration levels at test start and after 72 h.

Test solutions

Vehicle:
no
Details on test solutions:
100 mg/L of the test substance were weighed and mixed with dilution water by agitation.

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
22-23°C
pH:
test start: 8.09-8.18
test end: 8.13-9.53
Nominal and measured concentrations:
nominal: 10, 32, 100, 320 and 1000 µg/l
geometric mean of measured concentrations: 5.28, 9.28, 16.3, 30.1 and 395 µg/l
Details on test conditions:
With regard to the volatility of the test substance glass flasks without headspace were used to reduce losses of test item.
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC20
Effect conc.:
22.3 µg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95% CL 20.1 - 24.3
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
34.2 µg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95% CL 32.0 - 37.6
Results with reference substance (positive control):
EC50 (growth rateh, 72h): 0.69 mg/l (95% CL 0.64-0.74 mg/L)
Reported statistics and error estimates:
EC10-, EC20- and EC50-values of the growth rate and yield inhibition after 72 h were calculated by sigmoidal dose-response regression. Calculation of the confidence intervals EC10-, EC20- and EC50-values were carried out using standard procedures according to CLOPPER and PEARSON (1934).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes