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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline followed. Study well conducted.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
2007
Reference Type:
study report
Title:
Unnamed
Year:
2010

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: USEPA(2002) Methods for measuring the acute toxicity of effluents and receiving waters to freshwater organisms. Cincinnati, Ohio, U.S. Environmental Protection Agency
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Pb

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
- Test organisms originate from Aquatic Biosystems and maintained in dechlorinated tapwater at 26°C
- For all tests, a mass culture was started and maintained for 3 days. All mass cultures were fed with a combination of YCT and algae
- Test organisms were < 24 h old before testing

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
16.4 mg/L
Test temperature:
25°C
pH:
5.71+-0.05
Nominal and measured concentrations:
Nominal conc. (µg/L Pb):0, 5, 10, 20, 40, 80
Measured conc. (µg/L Pb): 0, 3, 9, 17, 36, 76
Details on test conditions:

- 5 individuals/20 mL polyethylene cups
- 4 replicates were used for testing

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
73.56 µg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
dissolved
Basis for effect:
mortality
Remarks on result:
other: 95%CI: (µg/L Pb2+): 45.1 - 119.1
Reported statistics and error estimates:
All acute tests were assessed for LC50 values using Maximum Likelihood Probit or Trimmed Spearman Karber method

Any other information on results incl. tables

Water quality parameters in micromol:

Na+: 78; Ca2 +: 106; Mg2 +: 58; K+: 55; Cl-: 408; SO42 -: 21; DOC: 50

Applicant's summary and conclusion