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Zebra fish were exposed to 2-allyloxymethyl-2-ethylpropanediol at nominal concentrations of 200, 400, 800, 1600 and 3200 mg/L in a semi-static 96 -hour acute toxicity test according to OECD 203 guidelines. The test design included an untreated control comprising of dilution water only. The 96-hour LC50 determined was 1200 mg/L (based nominal exposure concentrations). The NOEC (no-observed effect concentration) was 400 mg/L (Olsson, 2010).

Daphnia magna were exposed to 2-allyloxymethyl-2-ethylpropanediol at nominal loading rates of 100, 200, 400, 800, 1600 and 3200 mg/L in a static 48 -hour acute toxicity test according to OECD 202guidelines. The test design included an untreated control comprising of dilution water only. The 48-hour EC50 was 1530 mg/L (based nominal exposure concentrations). The NOEC (no-observed effect concentration) was 800 mg/L (Olsson, 2008).

The algae Pseudokirchneriella subcapitata was exposed to 2-allyloxymethyl-2-ethylpropanediol at nominal loading rates of 15.6, 31.2, 62.5, 125, 250, 500 and 1000 mg/L in a 72 -hour growth inhibition test according to the OECD 201 guideline. The test design included an untreated control comprising of dilution water only. The 72-hour ErC50 was 610 mg/L (based nominal exposure concentrations) and the corresponding NOEC (no-observed effect concentration) was 125 mg/L. The 72-hour EyC50 was 215 mg/L (based nominal exposure concentrations) and the corresponding NOEC (no-observed effect concentration) was 125 mg/L (Olsson, 2008).

The toxicity of trimethylolpropane diallyl ether (structural analogue to 2-allyloxymethyl-2-ethylpropanediol) to activated sewage sludge was investigated in a respiration inhibition test according to OECD test guideline 209. Following three hours exposure to trimethylolpropane diallyl ether the EC50 was observed to be > 1000 mg/L (based on nominal exposure concentrations). A 37% effect on respiration was seen at 1000 mg/L with no effect at 316 mg/L, therefore the NOEC was observed to 316 mg/L (Knight, TMPDE, 2010).

In the chemical safety assessment performed according to Article 14(3) in connection with Annex I section 3 (Environmental Hazard Assessment) no hazard was identified. Therefore according to REACH Annex I (5.0) exposure estimation is not necessary for long-term toxicity to fish and aquatic invertebrates. Consequently, in accordance with Column 2 of REACH Annex IX, the study does not need to be conducted as all identified uses of the substance are assessed as safe for the environment.