Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: OECD guideline conform

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
test concentration above water solubility
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
The test material is insoluble in water. A stock solution was prepared: 4.0 g of test material were dissolved in and made up to 10 ml with DMF. This solution was diluted to 100 mg/l with water.
Concentrations Nominal: The calculated amounts of stock solution to produce the desired test concentrations were given into the water and were homogeneously distributed. The daphnia were then transferred into the beakers.

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
- Concentrations: 10, 18, 32, 58 and 100 mg/l.

Test solutions

Vehicle:
not specified
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)

- Evidence of undissolved material (e.g. precipitate, surface film, etc): Small parts of the test substance were swimming at the surface of the test solutions at concentrations 10-100 mg/l (nominal) from the start of the test

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
not specified
Water media type:
not specified
Limit test:
no
Total exposure duration:
24 h

Test conditions

Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
> 100 mg/L
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Basis for effect:
mobility
Details on results:
No immobilization was noted in any treated organism.

Applicant's summary and conclusion

Conclusions:
Test concentrations were well above the limit of solubility. These test conditions may be considered to represent a worst-case exposure condition, as the possibility for both direct toxicity (narcosis) of dissolved substance and solvent vehicle, and indirect toxicity (physical effects) of undissolved test substance were represented.
Regardless of this potential for multiple modes of toxicity, acute EC50 value indicates the substance to be practically non-toxic to crustaceae on an acute basis.