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EC number: 257-182-1 | CAS number: 51410-72-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006-12-06 to 2006-12-21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study. GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Version / remarks:
- dated May 19, 2000
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- adopted 21 July 1997
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- (3-methacrylamidopropyl)trimethylammonium chloride
- EC Number:
- 257-182-1
- EC Name:
- (3-methacrylamidopropyl)trimethylammonium chloride
- Cas Number:
- 51410-72-1
- Molecular formula:
- C10 H21 N2 O. Cl
- IUPAC Name:
- trimethyl-[3-(2-methylprop-2-enoylamino)propyl]azanium chloride
- Test material form:
- other: aqueous solution
- Details on test material:
- - Name of test material (as cited in study report): Trimethylammoniumpropyl methacrylamide chloride
Constituent 1
Method
- Target gene:
- his
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Details on mammalian cell type (if applicable):
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 0.0316, 0.100, 0.316, 1.0, 2.5, 5 µL/plate (maximum spacing 3.16)
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: distilled water
- Justification for choice of solvent/vehicle: compatible with survival of bacteria and S9 activity
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- methylmethanesulfonate
- other: 4-nitro-o-phenylene-diamine (without metabolic activation)
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (with metabolic activation)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: plate incorporation / preincubation
DURATION
- Preincubation period: 60 min at 37°C
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: 3
NUMBER OF CELLS EVALUATED: colony count/plate
DETERMINATION OF CYTOTOXICITY
- Method: colony count
MUTATION FACTOR = (mean revertants; test item) / (mean revertants; solvent control) - Evaluation criteria:
- - colony count for spontaneous revertants on control plates without metabolic activation within historical range:
TA 98: 18 – 63
TA 100: 79 – 197
TA 1535: 5 – 30
TA 1537: 4 – 32
TA 102: 173 – 396
- positive controls show distinct enhancement of revertant rates over control plates
A test item is considered mutagenic if:
- a clear an d dose-related increase in revertant number occurs
- a biologically relevant positive response for at least one dose group occurs: in TA 100 and TA 102 number of revertants at least twice as high as in solvent control; TA 98, TA 1535, TA 1537 at least three times higher number of revertants as in solvent control - Statistics:
- not regarded necessary
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: no precipitation observed
COMPARISON WITH HISTORICAL CONTROL DATA: the number of spontaneous revertants was in the range of historical data for all strains
ADDITIONAL INFORMATION ON CYTOTOXICITY: no toxicity observed - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without metabolic activation
MAPTAC (50% in water) was investigated for its potential to induce gene mutations in Salmonella typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102 according to the plate incorporation test and the pre-incubation test in concentrations of 0.0316, 0.100, 0.316, 1.0, 2.5, 5 µL/plate.
No biologically relevant, dose-dependent increase in revertant numbers were observed in any tester strain at any concentration tested with and without metabolic activation.
MAPTAC is considered to be non-mutagenic in this bacterial reverse mutation assay. - Executive summary:
In a reverse gene mutation assay in bacteria according to OECD guideline 471, adopted 21 July 1997 and EU Method B.13/14, dated 19 May 2000, TA 98, TA 100, TA 1535, TA 1537, TA 102 strains of S. typhimurium were exposed to MAPTAC (50% in water) at concentrations of 0.0316, 0.100, 0.316, 1.0, 2.5, 5 µL/plate in the presence and absence of mammalian metabolic activation. The test was performed as plate incorporation test and as pre-incubation test.
Up to and including the highest concentration tested of 5 µL MAPTAC/plate, no signs of toxicity were observed. There was no evidence of induced mutant colonies over background.
The positive controls induced the appropriate responses in the corresponding strains.
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