1-(vinyloxy)octadecane

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989-01-19 to 1989-03-20

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study. Only four bacterial strains tested (S. typh. TA98, TA100, TA 1535, TA1537). No GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Version / remarks:

1988

Deviations:

no

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

1-(vinyloxy)octadecane

EC Number:

213-208-3

EC Name:

1-(vinyloxy)octadecane

Cas Number:

930-02-9

Molecular formula:

C20H40O

IUPAC Name:

1-(ethenyloxy)octadecane

Test material form:

other: colourless liquid

Method

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

Aroclor 1254-induced rat liver S-9 mix

Test concentrations with justification for top dose:

0; 20; 100, 500; 2500; 5000 µg/plate

Vehicle / solvent:

DMSO

Controls

Controls 1

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 2-Aminoanthracene (2-AA)

Remarks:

with S-9 mix; TA98, TA100,TA1535, TA1537

Controls 2

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)

Remarks:

without S-9 mix; TA100, TA1535

Controls 3

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 4-nitro-o-phenylenediamine

Remarks:

without S-9 mix; TA98

Controls 4

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

9-aminoacridine

Remarks:

without S-9 mix; TA1537

Details on test system and experimental conditions:

METHOD OF APPLICATION: Standard plate test
DURATION
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: 3 plates per dose and control

METHOD OF APPLICATION: Preincubation test
DURATION
- Preincubation period: 20 min at 37°C
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: 3 plates per dose and control

DETERMINATION OF CYTOTOXICITY
- Method: background growth

Evaluation criteria:

In general, a substance to be characterized as positive in the Ames test has to fulfill the following requirements:
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the results .

Statistics:

NA

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity nor precipitates, but tested up to recommended limit concentrations

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Additional information on results:

The slight but not dose-dependent increase in the number of his+ revertants using TA 1537 in the 1st experiment could not be confirmed either in a additional standard plate test or in a preincubation assay; therefore the findings of this 1st experiment must be regarded as incidental.
Toxicity: No bacteriotoxic effect (reduced his- background growth) was observed.
Solubility: Complete solubility of test substance in DMSO.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative