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EC number: 203-977-3 | CAS number: 112-49-2
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- Ecotoxicological Summary
- Aquatic toxicity
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- Short-term toxicity to fish
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- Long-term toxicity to aquatic invertebrates
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 Nov - 23 Jan 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- E. coli WP2 uvrA not tested
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,2-bis(2-methoxyethoxy)ethane
- EC Number:
- 203-977-3
- EC Name:
- 1,2-bis(2-methoxyethoxy)ethane
- Cas Number:
- 112-49-2
- Molecular formula:
- C8H18O4
- IUPAC Name:
- 2,5,8,11-tetraoxadodecane
- Reference substance name:
- Triglyme
- IUPAC Name:
- Triglyme
- Reference substance name:
- Triethylene glycol dimethyl ether
- IUPAC Name:
- Triethylene glycol dimethyl ether
- Details on test material:
- Purity: > 99.9%
colorless liquid
Constituent 1
Constituent 2
Constituent 3
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1538
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- Study I: 4, 20, 100, 500, 2500, 10000 µg/plate
Study II: 4, 20, 100, 500, 2500, 5000 µg/plate - Vehicle / solvent:
- distilled water
- Details on test system and experimental conditions:
- Toxicity experiments and dose range finding:
The first experiment (I) was performed with all tester strains using three plates per dose to get information on mutagenicity and toxicity for calculation of an approriate dose range. A reduced rate of spontaneously occuring colonies as well as visible thinning of the bacterial lawn were used as indicator for toxicity. Thinning of the bacterial lawn was controlled microscopically.
In combination with the second experiment, toxicity testing was performed as follows:
0.1 mL of the different dilutions of the test compound were thoroughly mixed with 0.1 mL of 10exp(-6) dilution of the overnight culture of TA100 and plate with Histidine and Biotin rich top agar (3 plates per dose). The solvent control is compared with the number of colonies per plate in the presence of the tst compound. Results are given as a ration of these values (=surviving fraction).
Mutagenicity test:
Top agar is prepared for the Salmonella strains by mixing 100 mL Agar (0.6 % Agar, 0.5% NaCl) with 10 mL of a 0.5 mL Histidine-biotin solution. The following ingredients are added to 2 mL of molten top agar at 46°C:
0.1 mL of an overnight nutrient broth culture of the bacterial tester strain, 0.1 mL test compound solution, 0.5 mL S9 mix or buffer.
After mixing, the liquid is pooured into a Petridish with minimal Agar (1.2 % Agar, Vogel-Bonner E medium with 2% Glucose). After incubation for approx. 48 hours at 37°C in the dark, colonies are counted. Two independent exeriments were performed.
Positive controls:
Positive control plates were included for each strain. the following substances were used as positive controls:
a) without S9 mix
Sodium azide: TA100, TA 1535
9-Aminocridine: TA1537
2-Nitrofluorene: TA98, TA1538
b) with S9 mix
Benzo[a]pyrene: TA98, TA100, TA1535, TA1537, TA1538
2-Aminoanthracene: TA98, TA100, TA1535, TA1537, TA1538 - Evaluation criteria:
- No data
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Controls:
Control plates (background control and positive controls) gave the expected number of colonies.
Toxcity test:
The test compound was tested at doses of 4 to 10000 µg/plate and proved to be not toxic to the bacterial strains. For mutagenicity testing 5000 µg/plate was chosen as the highest dose in the second experiment.
Mutagenicity test:
Triethylene glycol dimethyl ether did not cause a significant increase in the number of revertant colonies with any of the tester strains either in the absence or presence of S9 mix. No dose dependent effect was obtained. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
AmesTriethylene glycol dimethyl ether
Table 1: Summary of mean number of revertants (study I, mean of at 3 plates) in Salmonella typhimurium with and without metabolic activation (negative and positive controls)
Concentration |
TA 98 |
TA 100 |
TA1535 |
TA 1537 |
||||||||
[µg/plate] |
- S9 mix |
+ S9 mix |
- S9 mix |
+ S9 mix |
- S9 mix |
+ S9 mix |
- S9 mix |
+ S9 mix |
||||
0 |
22 |
28 |
189 |
201 |
13 |
14 |
8 |
7 |
||||
4 |
25 |
31 |
207 |
193 |
11 |
7 |
5 |
7 |
||||
20 |
23 |
27 |
194 |
217 |
12 |
8 |
8 |
6 |
||||
100 |
22 |
24 |
212 |
205 |
7 |
10 |
7 |
7 |
||||
500 |
20 |
27 |
212 |
206 |
11 |
10 |
3 |
7 |
||||
2500 10000 |
23 25 |
27 28 |
230 |
210 214 |
11 17 |
9 7 |
8 8 |
5 9 |
||||
|
|
|
|
|
|
|
|
|
||||
Positive: |
|
|
|
|
|
|
|
|
||||
Sodium Azide |
|
|
534 |
|
368 |
|
|
|
||||
9-Aminoacridine 2-Nitrofluorene |
635 |
|
|
|
|
|
135 |
|
||||
2-Aminoathracene |
|
585 |
|
841 |
|
122 |
|
120 |
||||
Benzo[a]pyrene |
|
731 |
|
1326 |
|
20 |
|
131 |
||||
|
|
|
|
|
|
|
|
|
||||
|
||||||||||||
Concentration |
TA 1538 |
|
|
|
||||||||
[µL/plate] |
- S9 mix |
+ S9 mix |
|
|
|
|
|
|
||||
0 |
14 |
12 |
|
|
|
|
|
|
||||
4 |
13 |
12 |
|
|
|
|
|
|
||||
20 |
10 |
18 |
|
|
|
|
|
|
||||
100 |
13 |
22 |
|
|
|
|
|
|
||||
500 |
18 |
20 |
|
|
|
|
|
|
||||
2500 10000 |
13 14 |
15 16 |
|
|
|
|
|
|
||||
|
|
|
|
|
|
|
|
|
||||
Positive: |
|
|
|
|
|
|
|
|
||||
Sodium Azide |
|
|
|
|
|
|
|
|
||||
9-Aminoacridine 2-Nitrofluorene |
725 |
|
|
|
|
|
|
|
||||
2-Aminoathracene |
|
362 |
|
|
|
|
|
|
||||
Benzo[a]pyrene |
|
199 |
|
|
|
|
|
|
||||
|
|
|
|
|
|
|
|
|
||||
Table 2: Summary of mean number of revertants (study II, mean of at 3 plates) in Salmonella typhimurium with and without metabolic activation (negative and positive controls)
Concentration |
TA 98 |
TA 100 |
TA1535 |
TA 1537 |
||||||||
[µg/plate] |
- S9 mix |
+ S9 mix |
- S9 mix |
+ S9 mix |
- S9 mix |
+ S9 mix |
- S9 mix |
+ S9 mix |
||||
0 |
19 |
29 |
191 |
195 |
12 |
9 |
11 |
10 |
||||
4 |
21 |
28 |
185 |
205 |
10 |
14 |
13 |
11 |
||||
20 |
19 |
29 |
181 |
193 |
11 |
11 |
12 |
9 |
||||
100 |
19 |
30 |
186 |
206 |
9 |
9 |
12 |
10 |
||||
500 |
17 |
32 |
195 |
203 |
9 |
10 |
9 |
13 |
||||
2500 5000 |
16 22 |
33 29 |
190 193 |
212 211 |
10 9 |
7 10 |
11 9 |
11 9 |
||||
|
|
|
|
|
|
|
|
|
||||
Positive: |
|
|
|
|
|
|
|
|
||||
Sodium Azide |
|
|
550 |
|
374 |
|
|
|
||||
9-Aminoacridine 2-Nitrofluorene |
739 |
|
|
|
|
|
106 |
|
||||
2-Aminoathracene |
|
598 |
|
1006 |
|
143 |
|
149 |
||||
Benzo[a]pyrene |
|
712 |
|
1508 |
|
31 |
|
156 |
||||
|
|
|
|
|
|
|
|
|
||||
|
||||||||||||
Concentration |
TA 1538 |
|
|
|
||||||||
[µL/plate] |
- S9 mix |
+ S9 mix |
|
|
|
|
|
|
||||
0 |
16 |
19 |
|
|
|
|
|
|
||||
4 |
14 |
15 |
|
|
|
|
|
|
||||
20 |
18 |
20 |
|
|
|
|
|
|
||||
100 |
16 |
23 |
|
|
|
|
|
|
||||
500 |
14 |
12 |
|
|
|
|
|
|
||||
2500 5000 |
11 14 |
16 15 |
|
|
|
|
|
|
||||
|
|
|
|
|
|
|
|
|
||||
Positive: |
|
|
|
|
|
|
|
|
||||
Sodium Azide |
|
|
|
|
|
|
|
|
||||
9-Aminoacridine 2-Nitrofluorene |
806 |
|
|
|
|
|
|
|
||||
2-Aminoathracene |
|
593 |
|
|
|
|
|
|
||||
Benzo[a]pyrene |
|
247 |
|
|
|
|
|
|
||||
|
|
|
|
|
|
|
|
|
||||
Applicant's summary and conclusion
- Conclusions:
Triethylene glycol dimethyl ether is not mutagenic in bacterial reverse mutation assay.- Executive summary:
Triethylene glycol dimethyl ether was tested for mutagenicity with the strains TA1538, TA1537, TA1535, TA100 and TA98 of Salmonella thyphimurium.
The mutagenicity study was conducted in the absence and presence of a metabolising system derived from rat liver homogenate. A concentration of 4 to 10000 µg Tirethylene glycol dimethyl ether/plate was used.
Control plates without mutagen did not show an increase in the number of revertant colonies. All positive control compounds gave the expected increase in the number of revertant colonies.
Triethylene glycol dimethyl ether did not show an increase in the number of revertants in any of the bacterial strains in the presence as well as in the absence of metabolic activation.
Therefore, it can be stated that Triethylene glycol dimethyl ether is not mutagenic in these bacterial test systems either with or without metabolic activation.
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