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Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1984-06-25 to 1984-07-02
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP study with analytics. Use of vehicle allowed testing of high concentrations well above the limit of water solubility (water solubility = 5-7 µg/L). Vehicle control showed no negative effects.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
adopted 12 May 1981
Deviations:
yes
Remarks:
: vehicle concentration > 100 mg/L (1900 mg/L)
GLP compliance:
no
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 100 mg/L (nominal); 57 mg/L (actual)
- Sampling time: at 0 h and 96 h exposure
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 1900 mg DMF; 0.4 mg Marlopon AT50 per L water
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none; the test substance appeared homogenously distributed at the test concentration
- Controls: blank and vehicle control
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: zebrafish
- Source: West-Aquarium / D-3422 Bad Lauterberg
- Length at study initiation (length definition, mean, range and SD): 25 - 33 mm (average: 28 mm)
- Weight at study initiation (mean and range, SD): 0.2 g (0.2 - 0.3 g)
- Feeding during test: none

ACCLIMATION
- Acclimation period: 159 d
- Acclimation conditions (same as test or not): same as test
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
152 mg CaCO3/L
Test temperature:
23 ± 1 °C
pH:
8.0
Dissolved oxygen:
80 - 99 %
Nominal and measured concentrations:
nominal: 100 mg/L
actual (geometric mean of measured concentrations at 0 h and 96 h): 57 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass aquaria of 20 L filled with 15 L (36/22/25 cm)
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.2 g/L

TEST MEDIUM / WATER PARAMETERS
- Watear: dechlorianted tap water
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH, O2, temperature - daily

OTHER TEST CONDITIONS
- Light: Fluorescent light, 16 h daily
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 57 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
> 57 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 57 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: none
- Mortality of control: none
- Mortalities vehicle: 10%
- Other adverse effects control: none
- Effect concentrations exceeding solubility of substance in test medium: yes
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1984-06-25 to 1984-07-02
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP study with analytics. Use of vehicle allowed testing of high concentrations well above the limit of water solubility (water solubility = 5-7 µg/L). Vehicle control showed no negative effects.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
adopted 12 May 1981
Deviations:
yes
Remarks:
: vehicle concentration > 100 mg/L (1900 mg/L )
GLP compliance:
no
Analytical monitoring:
yes
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Concentration of vehicle in test medium: 1900 mg DMF; 0.4 mg Marlopon AT50 per L water
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout
- Source: P. Hohler / CH-4314 Zeiningen
- Length at study initiation (length definition, mean, range and SD): 49 mm (47 - 50 mm)
- Feeding during test: none

ACCLIMATION
- Acclimation period: 27 d
- Acclimation conditions (same as test or not): same as test
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
152 mg CaCO3/L
Test temperature:
15 ± 1 °C
pH:
7.9-8.0
Dissolved oxygen:
73 - 98 % (during test)
Nominal and measured concentrations:
nominal: 100 mg/L
actual (geometric mean): 61 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass aquaria of 20 L filled with 15 L (36/22/25 cm)
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.3 g/L

TEST MEDIUM / WATER PARAMETERS
- Watear: dechlorianted tap water
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH, O2, temperature - daily

OTHER TEST CONDITIONS
- Light: Fluorescent light, 16 h daily
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 61 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
> 61 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 61 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: none
- Mortality of control: none
- Other adverse effects control: none
- Effect concentrations exceeding solubility of substance in test medium: yes

Description of key information

With high probability acutely not harmful to fish. 

Key value for chemical safety assessment

Additional information

In a 96-h acute toxicity study, zebra-fish (Brachydanio rerio) were exposed to test item at nominal concentrations of 0 (control, solvent control) and 100 mg/L (nominal) under static conditions. The 96-h LC50 was > 57 mg/L (average of measured concentration). A supporting study on rainbow trout according to OECD 203 did not reveal any toxic effects.

Neither of the studies revealed toxic effects in the range of water solubility.