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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1989
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
Identity: FAT 40'336/B
Batch: PP-Vers. 1/88-U0 33
Aggregate State: Powder
Solubility: Aqueous: >200 g/1 at 20 °C, Org.solv.: DMF, DMSO: >1000 mg/100 g at 20 °C
Stability: In aqueous medium: stable for at least 2 hours
Expiration Date: July 31, 1993
Storage: At room temperature, in the dark
Safety Precautions: Routine hygienic procedures were sufficient to assure personnel health and safety.
Analytical monitoring:
not specified
Vehicle:
no
Details on test solutions:
FAT 40336/B was dissolved in the test medium at a concentration of 100 mg/100 ml (clear blue stock dilution). Thereafter, series of sequential dilutions (which were based on pre-test* results) with the test medium were prepared to obtain the final test concentrations of the test article.
Test organisms (species):
Daphnia magna
Details on test organisms:
The toxicity study was run using the species Daphnia magna. The animals were bred in the laboratories of RCC under standardized conditions. For the tests, animals with an age of less than 24 hours were used.
Test type:
static
Water media type:
not specified
Limit test:
no
Total exposure duration:
24 h
Hardness:
14 °dH
Test temperature:
20 ± 2 °C
pH:
pH: Start: 7.4 - 8.5; End: 7.8 - 8.5
Dissolved oxygen:
The oxygen concentration and the pH was controlled in the test medium at the beginning and end of the respective experiments. The mean values found were: Oxygen: Start: 8.7 - 9.1 mg/L; End: 8.1 - 8.4 mg/L
Nominal and measured concentrations:
0, 62.5, 125, 250, 500 and 1000 mg/L (nominal)
Details on test conditions:
Not available
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
The EC50 value determined for potassium dichromate after 24 hours was 2.1 mg/L.
Validity criteria fulfilled:
yes
Conclusions:
The 24h EC50 of FAT 40'336/B was found to be >1000 mg/L.
Executive summary:

FAT 40'336/B was tested for its acute toxicity to Daphnia magna. The tests were run according to the Guideline 202, Part I of the OECD and to the Directive 84/449 EEC.

FAT 40'336/B was dissolved in the test medium at a concentration of 100 mg/100 ml (clear blue stock dilution). Thereafter, series of sequential dilutions with the test medium were prepared to obtain the final test concentrations of the test article. Along with the test article, control samples were run. As reference substance, potassium dichromate was used and the test was conducted. The Daphnia were exposed to the following test concentrations in two replicates (10 in each replicate) for 24 hours: 0, 62.5, 125, 250, 500 and 1000 mg/L.

The results are summarized as follows;

The EC0 (= NOEC) after 24 hours was determined to be 250 mg/l.

The EC100 value was found to be >1000 mg/l for 24 hours of exposure.

The EC50 value was estimated to be >1000 mg/l for the respective exposure period.

In conclusion, the 24h EC50 of FAT 40'336/B was found to be >1000 mg/L.

Description of key information

Currently no study to assess the short term toxicity of Reactive Blue 72 to aquatic invertebrates is available. However, a structurally similar substance, FAT 40336/B (Reactive blue 239) was tested for its acute toxicity to Daphnia magna. The tests were run according to the Guideline 202, Part I of the OECD and to the Directive 84/449 EEC. The Daphnia were exposed to the following test concentrations in two replicates (10 in each replicate) for 24 hours: 0, 62.5, 125, 250, 500 and 1000 mg/L.

The results are summarized as follows;

The EC0 (= NOEC) after 24 hours was determined to be 250 mg/l.

The EC100 value was found to be >1000 mg/l for 24 hours of exposure.

The EC50 value was estimated to be >1000 mg/l for the respective exposure period.

The 48h EC50 to Daphnia magna was considered to be >1000 mg/L.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
1 000 mg/L

Additional information