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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Report date:
2014

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
3-(3,4,5-trimethoxybenzylidene)pentane-2,4-dione
EC Number:
807-059-9
Cas Number:
945558-97-4
Molecular formula:
C15H18O5
IUPAC Name:
3-(3,4,5-trimethoxybenzylidene)pentane-2,4-dione
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
vehicle: acetone
Concentration of vehicle in test medium: 100ppm

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Group Size: 20 daphnids per group. Each group consisted of four replicates and 5 daphnids per replicate.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
204mg/L as CaCO3
Test temperature:
20.3±0.1℃
pH:
7.6±0.03
Dissolved oxygen:
8.59±0.05 mg/L
Nominal and measured concentrations:
25 mg/L
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Daphnids exposed to 25.0 mg Synoxyl HSS/L, control [without Synoxyl HSS] and vehicle control [Acetone] did not exhibit any abnormal behavioural symptoms at 24 and 48 h.
Results with reference substance (positive control):
EC50: 0.85 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
the study was conducted as a limit test, the 48h EC50 of Synoxyl HSS was greater than 25 mg/L.