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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Report date:
2014

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
3-(3,4,5-trimethoxybenzylidene)pentane-2,4-dione
EC Number:
807-059-9
Cas Number:
945558-97-4
Molecular formula:
C15H18O5
IUPAC Name:
3-(3,4,5-trimethoxybenzylidene)pentane-2,4-dione

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
other: Secondary effluent of a treatment plant
Details on inoculum:
The Secondary effluent of a treatment plant was collected from Sewage Treatment Plan, JRF. then filtered through Whatman No 1 filter paper and incubated at test temperature 20±1℃ in incubator for 6 days in dark.
Duration of test (contact time):
ca. 28 d
Initial test substance concentration
Initial conc.:
ca. 2 mg/L
Based on:
formulation
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance
Reference substance:
other: potassium hydrogen phthalate

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
ca. -2.04
Sampling time:
7 d
Parameter:
% degradation (O2 consumption)
Value:
ca. 2.3
Sampling time:
14 d
Parameter:
% degradation (O2 consumption)
Value:
ca. 8.18
Sampling time:
21 d
Parameter:
% degradation (O2 consumption)
Value:
ca. 30.91
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
the degradation for reference substance (potassium hydrogen phthalate) on day 7, 14, 21 and 28 were 61.70, 79.15, 90.64 and 82.55%, respectively.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Interpretation of results:
other: not readily biodegradable
Conclusions:
The Synoxyl HSS did not inhibit degradation of reference substance by more than 25% after 14 days, means no adverse effects of Synoxyl HSS on the inoculum. Therefore, Synoxyl HSS is not readily biodegradable.