Registration Dossier
Registration Dossier
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A mixture of: 4-(2,2,3-trimethylcyclopent-3-en-1-yl)-1-methyl-2-oxabicyclo[2.2.2]octane; 1-(2,2,3-trimethylcyclopent-3-en-1-yl)-5-methyl-6-oxabicyclo[3.2.1]octane; spiro[cyclohex-3-en-1-yl-[(4,5,6,6a-tetrahydro-3,6',6',6'a-tetramethyl)-1,3'(3'aH)-[2H]cyclopenta[b]furan]; spiro[cyclohex-3-en-1-yl-[4,5,6,6a-tetrahydro-4,6',6',6'a-tetramethyl)-1,3'(3'aH)-[2H]cyclopenta[b]]furan]
EC number: 422-040-1 | CAS number: 426218-78-2 CASSIFFIX
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The toxicity of the substance to aquatic algae was examined in a study according to OECD TG 201, and in compliance with GLP (Huntingdon, 1995). In this study, cultures of freshwater green algae (P. subcapitata) were exposed to nominal test concentrations of 0 (control), 3.125, 6.25, 12.5, 25 and 50 mg/L for 72 hours. The test compound was dispersed in 20% Tween 80 acetone to give an initial stock solution of 500 mg/mL and subsequent dilution of this stock with auxiliary solvent gave a series of stock solutions from which the desired test exposure levels were prepared. Test substance analysis was performed (samples not filtered to remove algal cells prior to analysis) and measured concentrations were found to range from 77 - 91 % of nominal at 0 hours and 19 - 43% of nominal at 72 hours. Geometric mean measured concentrations were calculated to be 0, 1.2, 2.6, 7.8, 13 and 31 mg/L. Algal cell densities were determined at 0, 24, 48 and 72 hours by direct counting, and inhibition of algal growth calculated.
No significant effect on growth rate was observed at the test concentrations of 1.2 and 2.6 mg/L. At test concentrations of 7.8, 13 and 31 mg/L inhibition of growth rate was determined to be 10%, 50% and 63%, respectively. There were no abnormalities detected in the control and test cultures except at 7.8, 13 and 31 mg/L treatment levels where the cells appeared turgid. No cultures showed any signs of contamination by foreign algal cells or protozoa. Based on these findings, the 72 -h ErC50 and NOErC values were determined at 13 mg/L and 2.6 mg/L, respectively, based on geometric mean measured concentrations.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 13 mg/L
- EC10 or NOEC for freshwater algae:
- 2.6 mg/L
Additional information
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