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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

As there is no data available for tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate , results of the parent acid, 2-phosphonobutane-1,2,4-tricarboxylic acid are taken into account for this endpoint.
A LC50 (96h) of > 1042 mg/L was concluded from the results of the available prolonged toxicity study in fish.

Key value for chemical safety assessment

LC50 for freshwater fish:
1 042 mg/L

Additional information

As there is no data available for tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate a read-across approach with the corresponding parent acid 2-phosphonobutane-1,2,4-tricarboxylic acid is proposed.

In aqueous media, tetrasodium hydrogen 2-phosphonatobutane-tricarboxylate and 2-phosphono-butane-1,2,4-tricarboxylic acid dissociate into the corresponding anion (2-phosphonatobutane-tricarboxylate ion) and the sodium ion and hydrogen ion (proton), respectively. Fate, behavior and the ecotoxicological properties of 2-phosphonobutane-1,2,4-tricarboxylic acid and its tetrasodium salt are thought to be an effect of the phosphonato-carboxylate ion rather than of the sodium ion or the hydrogen ion (proton), which are normal constituents in environmental systems and have no relevant ecotoxic properties in low concentrations.

Therefore a read-across between tetrasodium hydrogen 2-phosphonatobutane-tricarboxylate and 2-phosphonobutane-1,2,4-tricarboxylic acid is justified.

Prolonged toxicity of 2-phosphonobutane-1,2,4-tricarboxylic acid to fish (Danio rerio) has been tested under semistatic conditions in accordance to OECD Guideline 204 for an exposure period of 14 days. No mortality was recorded for this prolonged toxicity study in 10 fishes (species: zebra danio) per dose level. Behaviour and development of length and body weight was comparable to those of the controls. A NOEC of >/= 1042 mg/l is obtained in this test. The result is interpreted as 14 d-LC50 of > 1042 mg/l referring to measured concentrations, as the test concentrations were confirmed by analytical monitoring using ion chromatography.

This toxicity study is classified as acceptable and satisfies the guideline requirements for the toxicity study to fish (Danio rerio).