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EC number: 272-939-6 | CAS number: 68921-42-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- experimental data of read across substances
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on four toxicity to microorganism studies as-
WoE 2 and WoE 3. - GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Details on sampling:
- WoE 2 and WoE 3:
- Concentrations: Test chemical concentration used for the study was 0.1% (1000 mg/l) and 1% (10000 mg/l), respectively. - Vehicle:
- not specified
- Test organisms (species):
- Paramaecium caudatum
- Details on inoculum:
- WoE 2 and WoE 3:
- Other: Cultures of Paramecium caudatum (PC): PC was maintained at 22°C on 0.15 % dried lettuce infusion and fed with Aerobacter aerogenes. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 20 min
- Test temperature:
- WoE 2 and WoE 3: 22°C
- pH:
- WoE 2 and WoE 3: 7.0
- Details on test conditions:
- WoE 2 and WoE 3:
TEST SYSTEM
- Test vessel: Slide glass
- Biomass loading rate: 30 to 40 Paramaecium caudatum/conc.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The mean survival time and the death rate were calculated. The survivial time was defined as the time required until death was observed for each concentration. Death was assumed to have occurred when there was no movement. The death rate was defined as the precentage of deaths observed during 20 min. - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 20 min
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Mortality
- Remarks on result:
- other: WoE 2
- Key result
- Duration:
- 20 min
- Dose descriptor:
- other: EC
- Effect conc.:
- 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Mortality
- Remarks on result:
- other: WoE 2: Death rate = 63.3%
- Key result
- Duration:
- 20 min
- Dose descriptor:
- other: EC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Mortality
- Remarks on result:
- other: WoE 3: Death rate = 36.7%
- Validity criteria fulfilled:
- not specified
- Conclusions:
- On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, the 20 mins NOEC value can be expected to be 1000 mg/l and EC50 value can be expected to be in the range 1000 to 10000 mg/l, respectively.
- Executive summary:
Data available of the structurally and functionally similar read across chemicals has been reviewed to determine the effect of the test chemical on toxicity to microorganisms. The studies are as mentioned below:
Toxicity to microorganisms study was carried out for 20 mins. Paramaecium caudatum (Pc) was used as a test organism. Test organism was maintained at 22°C on 0.15 % dried lettuce infusion and fed with Aerobacter aerogenes. Test organisms were exposed to chemical conc. (0.1% (1000 mg/l) and 1% (10000 mg/l)) in a hollow glass slide. An equal volume of 0.04 M phosphate buffer (pH 7.0) was added. in the test vessel. After 5 to 10 Pc were added, their survival time were measured microscopically. 30 to 40 Paramaecium caudatum/conc. were tested by the same method. After an exposure period of 20 mins, the mean survival time and the death rate were calculated. The survival time was defined as the time required until death was observed for each concentration. Death was assumed to have occurred when there was no movement. The death rate was defined as the percentage of deaths observed during 20 min. On the basis of the effect of test chemical on mortality of the test organism Paramaecium caudatum, the 20 mins NOEC and EC value was determined to be 1000 and 10000 mg/l, respectively.
Another toxicity to microorganisms study was carried out for 20 mins. Paramaecium caudatum (Pc) was used as a test organism. Test organism was maintained at 22°C on 0.15 % dried lettuce infusion and fed with Aerobacter aerogenes. Study was performed using the same method as mentioned in above study. On the basis of the effect of test chemical on mortality of the test organism Paramaecium caudatum, the 20 mins EC value was determined to be 1000 mg/l.
On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, the 20 mins NOEC value can be expected to be 1000 mg/l and EC50 value can be expected to be in the range 1000 to 10000 mg/l, respectively.
Reference
WoE 2:
Table: Effect of food dyes on the mean survival time and death rate of Paramecium caudatum.
Food dyes |
Test chemical concentration |
|||
1.0% |
0.1% |
|||
Mean survival time (sec) |
Death rate (%) |
Mean survival time (sec) |
Death rate (%) |
|
Test chemical |
881 |
63.3 |
No deaths were observed for 20 mins |
7.7 |
WoE 3:
Table:Effect of food dyes on the mean survival time and death rate of Paramecium caudatum.
Food dyes |
Test chemical concentration |
|||
1.0% |
0.1% |
|||
Mean survival time (sec) |
Death rate (%) |
Mean survival time (sec) |
Death rate (%) |
|
Test chemical |
Impossible to observe microscopically because of high concentration. |
Impossible to observe microscopically because of high concentration. |
1037 |
36.7 |
Description of key information
On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, the 20 mins NOEC value can be expected to be 1000 mg/l and EC50 value can be expected to be in the range 1000 to 10000 mg/l, respectively.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
Data available of the structurally and functionally similar read across chemicals has been reviewed to determine the effect of the test chemical on toxicity to microorganisms. The studies are as mentioned below:
Toxicity to microorganism study was carried out for 20 mins. Paramaecium caudatum (Pc) was used as a test organism. Test organism was maintained at 22°C on 0.15 % dried lettuce infusion and fed with Aerobacter aerogenes. Test organisms were exposed to chemical conc. (0.1% (1000 mg/l) and 1% (10000 mg/l)) in a hollow glass slide. An equal volume of 0.04 M phosphate buffer (pH 7.0) was added. in the test vessel. After 5 to 10 Pc were added, their survival time were measured microscopically. 30 to 40 Paramaecium caudatum/conc. were tested by the same method. After an exposure period of 20 mins, the mean survival time and the death rate were calculated. The survival time was defined as the time required until death was observed for each concentration. Death was assumed to have occurred when there was no movement. The death rate was defined as the percentage of deaths observed during 20 min. On the basis of the effect of test chemical on mortality of the test organism Paramaecium caudatum, the 20 mins NOEC and EC value was determined to be 1000 and 10000 mg/l, respectively.
Another toxicity to microorganism study was carried out for 20 mins. Paramaecium caudatum (Pc) was used as a test organism. Test organism was maintained at 22°C on 0.15 % dried lettuce infusion and fed with Aerobacter aerogenes. Study was performed using the same method as mentioned in above study. On the basis of the effect of test chemical on mortality of the test organism Paramaecium caudatum, the 20 mins EC value was determined to be 1000 mg/l.
On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, the 20 mins NOEC value can be expected to be 1000 mg/l and EC50 value can be expected to be in the range 1000 to 10000 mg/l, respectively.
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