2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

yes

Test type:

acute toxic class method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether

EC Number:

200-076-7

EC Name:

2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether

Cas Number:

51-03-6

Molecular formula:

C19H30O5

IUPAC Name:

2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

Source : Buckshire Corp. Perkasie, PA 18944, USA.
Age/weight at study initiation : 200-293 g
Number of animals per group : Five rats per test group
Control animals : No

The animals were housed and maintained in accordance with standards set forth in the Guide for the care and use of laboratory animals. The rats were acclimated to the laboratory for at least 7 days prior to dosing. Animals were individually identified by ear punch. Each cage was identified with a cage card displaying the project number, sex, date dosed, and responsible technician's initials.

Husbandry Conditions
Temperature: 19 -25°C.
Relative Humidity: 40 -70%.
Light: 12hour light/dark cycle.
Diet: ad libitum. Based on our current knowledge, no contaminants are known to be in this diet or water which might be expected to interfere with the objectives of the study.
Caging: Stainless steel elevated wire mesh flooring, 1 rat/cage

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Doses:

2.01, 3.01, 3.70, 5.00, 7.50 and 11.25 g/kg bw

No. of animals per sex per dose:

50

Control animals:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

5 630 mg/kg bw

Clinical signs:

other: Clinical signs of intoxication were yellow anogenital staining, ruffled fur, lethargy and sometimes dark nasal (and ocular) staining and ruffled skin.

Gross pathology:

Gross pathological examination of animals which died showed a dark liquid in the lower gastrointestinal tract. Mainly at the higher dosage groups additional changes like clear or dark muzzle staining, haemorrhagic lungs, yellow genital staining, pale liver and/or kidney and gas in the lower gastrointestinal tract were observed in one or the other case.

Any other information on results incl. tables

NOEL for clinical signs is 2.01 g/kg b.w.

Acute oral LD₅₀:

male rats as                         4.57 g/kg (3.65 – 5.73 g/kg)

female rats as                     7.22 g/kg (4.94 – 10.55 g/kg)

rats (both sexes) as            5.63 g/kg ( 4.40 – 7.2 g/kg)

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The test article, when administered as supplied to male albino rats, has an acute oral LD50 of 4.57g/Kg of active ingredent with 95% confidence limits from 3.65 to 5.73 g/Kg of active ingredient.
The test article, when administered as supplied to female albino rats, has an acute oral LD50 of 7.22 g/Kg of active ingredent with 95% confidence limits from 4.94 to 0.55 g/Kg of active ingredient.
The test article, when administered as supplied to male and female albin rats combined, has an acute oral LD50 of 5.63 g/Kg of active ingredent with 95% confidence limits from 4.40 to 7.20 g/Kg of activee ingredient.
The no effect level for clinical signs is 2.01 g/Kg.
Piperonyl ^Butoxide is of low acute oral toxicity.