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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1992
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
EPA OPP 72-2 (Aquatic Invertebrate Acute Toxicity Test)
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
yes
Details on test solutions:
vehicle methanol 0.01% v/v
Test organisms (species):
Daphnia magna
Details on test organisms:
Test material FEP 100 Piperonyl Butoxide Task Force II Blend 5 x 1 mCi 14C- Piperonyl Butoxide
Lot/Batch number Nonradiolabelled: 12/12/89 Radiolabelled: WIL 2118-E
Purity Nonradiolabelled: 90.78 % Radiolabelled: 99.9 %
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
143 mg/L as CaCO3 (range 140-148, n=4)
pH:
8.1 (range 8.0 – 8.2; n=4)
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
150 µg/L
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
not specified
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
510 µg/L
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
not specified
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 740 µg/L
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
not specified

For the treated groups with nominal PBO concentrations of0.13, 0.22, 0.36, 0.6 and 1.0 ppmthe mean measured concentrations of radioactivity were 0.08, 0.15, 0.25, 0.4 and 0.74 ppm, respectively. In the solvent control group all animals appeared healthy and normal throughout the test. Signs of abnormal behaviour among daphnia exposed to PBO at 0.40 and 0.74 ppm were observed after 48 hours. At these concentrations all of the organisms were discoloured and some organisms started to be lethargic.

No organisms died or were immobile at PBO concentration levels of 0.08 and 0.15 ppm. After 48 h one daphnid was dead in the treated group exposed to 0.25 ppm. At concentration levels of 0.40 ppm 30 % of the daphnia were immobile after 48 h. At a concenrtration level of 0.74 ppm, 50 % of the daphnids were immobile and 30 % were dead at the end of the test.

Validity criteria fulfilled:
yes
Conclusions:
The results of this study demonstrated a no mortality/immobilisation concentration of PBO for daphnia of 0.15 ppm (150µg/L) and an EC50 value of 0.51 ppm (510 µg/L) after 48 h exposure.
Based on the results of this study (see below) Piperonyl Butoxide would be classified as very highly toxic to Daphnia magna.

Description of key information

The results of this study demonstrated a no mortality/immobilisation concentration of PBO for daphnia of 0.15 ppm (150µg/L) and an EC50 value of 0.51 ppm (510 µg/L) after 48 h exposure.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
510 µg/L

Additional information

Based on the results of the studies provided Piperonyl Butoxide would be classified as very highly toxic to Daphnia magna.