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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1992
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Qualifier:
according to
Guideline:
EPA OPP 72-1 (Fish Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
yes
Test organisms (species):
Cyprinodon variegatus
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
21.0 – 22.5 °C
pH:
8.1 – 8.3
Dissolved oxygen:
5.8 – 7.6 mg/L
Details on test conditions:
Initial concentrations of test substance 1.3, 2.2, 3.6, 6.0 and 10.0 ppm of PBO
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
2.97 mg/L
Remarks on result:
other: (measured/nominal)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
3.94 mg/L
Remarks on result:
other: (calculated)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
>= 5.24 mg/L
Remarks on result:
other: (measured/nominal)

For the treated groups with nominal PBO concentrations of 1.3, 2.2, 3.6, 6.0 and 10.0 ppm the mean measured concentrations of radioactivity were 0.92, 1.84, 2.97, 5.24 and 6.62 ppm, respectively. In the solvent control group all animals appeared healthy and normal throughout the test. Signs of abnormal behaviour among fish exposed to PBO at 1.84 ppm were observed by 72 hours. At concentrations greater than or equal to 2.97 ppm effects were noted within approximately two hours of test initiation. By the end of the test all of the fish at concentrations greater than 2.97 ppm were dead or exhibited signs of abnormal behaviour.No fish died at PBO concentration levels of 0.92, 1.84 and 2.97 ppm. At the PBO concentration level of 5.24 ppm the percent mortalities by 48, 72 and 96 hours of exposure were 20, 80 and 100 %. At the highest exposure level of 6.62 ppm the percent mortalities were 25, 90 and 100 % after 24, 48 and 72 hours of exposure.

Validity criteria fulfilled:
yes
Conclusions:
The results of this study demonstrated that the 96 h no mortality concentration of PBO for sheepshead minnows was 2.97 ppm. The 96 h LC50 of PBO was 3.94 ppm (3.94 mg/L).

Description of key information

For sheepshead minnows the 96 h LC50 of PBO was 3.94 ppm (3.94 mg/L).

For bluegill the 96 h LC50 of PBO was 5.37 ppm (5.37 mg/L).

For Rainbow Trout (Oncorhynchus mykiss) the LC50 96h was 6.12 mg/l.

Key value for chemical safety assessment

LC50 for freshwater fish:
3.94 mg/L

Additional information