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Toxicity to microorganisms

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Two guideline studies on the respiration and growth of microorganisms respectively are available. A study conducted in compliance with DIN 38412, part 8 investigated the growth inhibition induced by the substance in Pseudomonas putida. An EC50 of 11 mg/l (EC10: 6.7 mg/l) was determined after an inoculation time of 17 hours (BASF AG, 1989). In a second study following guideline ISO 8192, the effect of the substance on the respiration rate of industrial activated sludge was determined. The test was terminated after 30 minutes inoculation time. The study reports an EC10 > 2000 mg/l (BASF AG, 1989).