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Diss Factsheets

Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with restrictions (limited documentation, 4 h exposure, occlusive treatment, limit test with only 1884 mg/kg bw and 5 animals)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1963
Report date:
1963

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Deviations:
yes
Remarks:
(limited documentation, 4 h exposure, occlusive exposure, limit dose 1884 mg/kg bw instead of 2000, only 5 animals tested)
GLP compliance:
no
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Suberonitrile
EC Number:
211-089-2
EC Name:
Suberonitrile
Cas Number:
629-40-3
Molecular formula:
C8H12N2
IUPAC Name:
octanedinitrile
Details on test material:
- Name of test substance (as cited in study report): Korksaeuredinitril
- Analytical purity: no data
- Physical state: liquid
- Density: 0.942 g/mL

Test animals

Species:
rat
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 131 g (mean)

No additional information reported.

Administration / exposure

Type of coverage:
occlusive
Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
TEST SITE
- Area of exposure: 30 cm²
- Type of wrap if used: no wrap; animals were placed in a bath tub filled with the test substance

REMOVAL OF TEST SUBSTANCE
- Washing (if done): Lutrol (conc.)
- Time after start of exposure: 4 h

TEST MATERIAL
- Amount(s) applied: 2 mL of the pure test substance
Duration of exposure:
4 h
Doses:
2000 µg/kg bw (corresponding to approx. 1884 mg/kg bw; calculated assuming test substance density = 0.942 g/mL)
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: no data
- Frequency of weighing: before application and at the end of the study
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
LD0
Effect level:
1 884 mg/kg bw
Based on:
test mat.
Remarks on result:
other: calculated assuming test substance densitiy = 0.942 g/mL
Mortality:
0/5 animals died
Clinical signs:
other: No clinical signs of toxicity were observed.
Gross pathology:
No abnormalities were observed.
Other findings:
No symptoms of local irritation were observed.

Applicant's summary and conclusion