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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Principles of method if other than guideline:
Acute Immobilization Study of test material on Daphnia magna in a Semi-Static System for 48 hrs. Test performed according to the OECD guideline 202.
GLP compliance:
no
Analytical monitoring:
not specified
Vehicle:
no
Details on test solutions:
The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of ADaM’s media. Achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L, and 100 mg/L, respectively.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
24 and 48 hrs
Hardness:
145.5 mg of CaCO3
Test temperature:
20±2
pH:
7.3
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):
not specified
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

9.1. Immobility

Nominal

 

Number

of

Immobility

 

 

Concentration

Daphnids

0 h

24 h

 

48 h

(mg/l)

 

 

 

 

 

 

 

Control

 

10

-

-

 

 

-

6.25

 

10

-

-

 

 

-

12.5

 

10

-

-

 

 

-

25

 

10

-

-

 

 

-

50

 

10

-

-

 

 

-

100

 

10

-

-

 

 

-

9.2. pH and oxygen content:

Nominal

 

Number of

pH(mg/L)

 

Concentration

daphnids

0h

24h

48h

(mg/l)

 

 

 

 

 

Control

 

10

7.27

7.39

7.39

6.25

 

10

7.41

7.50

7.50

12.5

 

10

7.49

7.55

7.55

25

 

10

7.53

7.61

7.61

50

 

10

7.55

7.63

7.63

100

 

10

7.60

7.69

7.69

Nominal

 

Number of

DO(mg/L)

 

Concentration

daphnids

0h

24h

48h

(mg/l)

 

 

 

 

 

Control

 

10

6.9

6.8

6.8

6.25

 

10

6.9

6.7

6.7

12.5

 

10

6.9

6.7

6.7

25

 

10

6.9

6.7

6.7

50

 

10

6.9

6.7

6.7

100

 

10

6.9

6.7

6.7

9.3. Test temperature:

Nominal

 

Number of

Temparature

 

 

 

Concentration

daphnids

0h

24h

48h

(mg/l)

 

 

 

 

 

Control

 

10

21.9

21.8

21.8

6.25

 

10

21.9

21.8

21.8

12.5

 

10

21.9

21.8

21.8

25

 

10

21.9

21.8

21.8

50

 

10

21.9

21.8

21.8

100

 

10

21.9

21.8

21.8

Validity criteria fulfilled:
yes
Remarks:
1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels
Conclusions:
Based on nominal concentrations, experimental median effective Concentrations [EC-0 (48 h)] for test substance on test daphnia was found to be 100 mg/L.
Executive summary:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test material. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of ADaM’s media. Achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L, and 100 mg/L, respectively. Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC0) for test material on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be 100 mg/l. Thus, on the basis of this EC0 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, does not exhibit short term toxicity to Daphnia.

Based on the EC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

Description of key information

Short-term toxicity to aquatic invertebrates:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test material. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of ADaM’s media. Achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L, and 100 mg/L, respectively. Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC0) for test material on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be 100 mg/l. Thus, on the basis of this EC0 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, does not exhibit short term toxicity to Daphnia.

Based on the EC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
50 mg/L

Additional information

Short-term toxicity to aquatic invertebrates:

Effect of test material was observed on aquatic invertebrate in an experimental report along with the data from peer reviewed journal.

In experimental report ,daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test material. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of ADaM’s media. Achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L, and 100 mg/L, respectively. Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC0) for test material on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be 100 mg/l. Thus, on the basis of this EC0 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, does not exhibit short term toxicity to Daphnia.

Based on the EC50, it can be consider that the chemical was not toxic and can be consider to be not classified as per the CLP classification criteria.

In peer reviewed journal ,the acute toxicity was determined according to the OECD guideline 202 (48 h acute immobilization assay). After 48 h, motile and immobilized D. magna were counted. The LC50 value in Daphnia magna was observed to be 50 mg/l after 48 h.Test substance is redialy degradable in aquatic environment , hence test substance is considered to be not classified as per CLP criteria.