Sulisobenzone

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

fish, juvenile growth test

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: from peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: Fish in vivo screening assay (OECD, 2004).

Principles of method if other than guideline:

Fish, juvenile growth test in vivo screening assay (OECD, 2004) was performed.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

Stock solutions were made in ethanol and stored in the dark at 4C.

Test organisms (species):

Pimephales promelas

Details on test organisms:

Juvenile, sexually undifferentiated fathead minnows (Pimephales promelas), between 2 and 3 months of age and with a total body length between 19 and 27 mm

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

14 d

Hardness:

total hardness 160 mg/l as CaCO3

pH:

7.2 - 7.9

Dissolved oxygen:

6.5–8.3 mg/l

Nominal and measured concentrations:

Nominal concentrations-10, 1000, 5000 ug/l.

Details on test conditions:

total alkalinity 30 mg/l as CaCO3; solvent control (SC, 1 ml ethanol in 10 liters of water) and positive control for estrogenic activity (100 ng/l E2)

Reference substance (positive control):

yes

Remarks:

100 ng/l E2 (17β-Estradiol)

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

1.048 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: body size

Remarks on result:

other: Actual value- 1048 µg/L

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

> 4.897 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

dissolved

Basis for effect:

mortality

Remarks on result:

other: Actual value- 4897 µg/L

Results with reference substance (positive control):

No mortality was observed in positive control

Conclusions:

Long term toxicity (14 d) value of test material to Pimephales promelas examined of NOEC:1.048 mg/l (on the basis of body size) and NOEC: >4.897 mg/l (on the basis of mortality).

Executive summary:

The 14-days fish experiments were conducted using juvenile, sexually undifferentiated fathead minnows (Pimephales promelas), between 2

and 3 months of age and with a total body length between 19 and 27 mm . Fish were held in well-aerated reconstituted tap water medium.

The studies were conducted using a 24-h static-renewal procedure with daily renewal of total aquaria water. For exposure, 10 randomly selected fish were each placed in stainless-steel tanks (10 liter) and exposed to individual UV filters for 14 days. Long term toxicity (14 d) value of test material to Pimephales promelas examined of NOEC:1.048 mg/l (on the basis of body size) and NOEC: >4.897 mg/l (on the basis of mortality). Based on the above effect concentration it is considered to be non - hazardous to aquatic environment and can not be classified as per CLP criteria .

Description of key information

Long term toxicity to fish:

The 14-days fish experiments were conducted using juvenile, sexually undifferentiated fathead minnows (Pimephales promelas), between 2

and 3 months of age and with a total body length between 19 and 27 mm . Fish were held in well-aerated reconstituted tap water medium.

The studies were conducted using a 24-h static-renewal procedure with daily renewal of total aquaria water. For exposure, 10 randomly selected fish were each placed in stainless-steel tanks (10 liter) and exposed to individual UV filters for 14 days. Long term toxicity (14 d) value of test material to Pimephales promelas examined of NOEC:1.048 mg/l (on the basis of body size) and NOEC: >4.897 mg/l (on the basis of mortality). Based on the above effect concentration it is considered to be non - hazardous to aquatic environment and can not be classified as per CLP criteria .

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

4.897 mg/L

Additional information

Long term toxicity to fish:

The 14-days fish experiments were conducted using juvenile, sexually undifferentiated fathead minnows (Pimephales promelas), between 2

and 3 months of age and with a total body length between 19 and 27 mm . Fish were held in well-aerated reconstituted tap water medium.

The studies were conducted using a 24-h static-renewal procedure with daily renewal of total aquaria water. For exposure, 10 randomly selected fish were each placed in stainless-steel tanks (10 liter) and exposed to individual UV filters for 14 days. Long term toxicity (14 d) value of test material to Pimephales promelas examined of NOEC:1.048 mg/l (on the basis of body size) and NOEC: >4.897 mg/l (on the basis of mortality). Based on the above effect concentration it is considered to be non - hazardous to aquatic environment and can not be classified as per CLP criteria .