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EC number: 421-450-8 | CAS number: 154702-15-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- A mixed population of activated sewage sludge micro-organisms was obtained for the range-finding test and for the definitive test from the aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK .
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Hardness:
- Laboratory tap water dechlorinated
140 mg/L as CaCO3 - Test temperature:
- about 21 °C
- pH:
- 8.1 at the start of the experiment
8.5 at the end of the experiment - Reference substance (positive control):
- yes
- Remarks:
- 3.5 -dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- inhibition of total respiration
- Validity criteria fulfilled:
- yes
- Conclusions:
- The effect of the test material on the respiration of activated sewage sludge micro-organisms gave a 3-h EC50 > 1000 mg/L. The NOEC after 3 h exposure was 1000 mg/L.
Reference
Range-finding Test
No significant effect on respiration was observed at either test concentration employed. Based on this information, a single test concentration, in triplicate, of 1000 mg/L was selected for the definitive test. The test material was insoluble in water and it was justifiable to conduct the definitive test at a single concentration of 1000 mg/L in excess of the water solubility level of the test material.
Definitive test
It was considered unnecessary and unrealistic to test at concentrations in excess of 1000 mg/L. The initial and final dissolved oxygen concentrations were below those recommended in the test guidelines (6.5 and 2.5 mg O2/L respectively). This was considered to have had no adverse effect on the results of the study given that in all cases the oxygen consumption rate was determined over the linear portion of the oxygen consumption trace. The validation criteria for the control respiration rates have been satisfied. Observations made showed that at the test concentration of 1000 mg/L at 0 hours and 30 mins contact time there was a dark brown dispersion with particles of test material on the surface and dispersed throughout. A pale brown dispersion with particles of test material dispersed homogenously throughout was observed at 3 hours contact time.
Description of key information
3-h NOEC = 1000 mg/L (nominal concentrations), activated sludge, respiration inhibition, OECD TG 209 and EU method C.11, 2008
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
A GLP limit study on the respiration inhibition of activated sewage sludge is available for this endpoint. The study followed the OECD TG 209 and the EU method C.11 (Reliability 1). The activated sludge was exposed in triplicate to a control and a nominal test concentration of 1000 mg/L for 3 hours. In addition, 3,5-dichlorophenole was used as a positive control. The test conditions were: temperature about 21 °C, pH 8.1-8.5. The 3-h test substance NOEC was determined to be 1000 mg/L based on nominal concentrations.
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