o-phenylenediamine

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods

Remarks:

As per ICCA HPV review conducted by Haskell.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 40 CFR Part 797.1330 Daphnid chronic toxicity test. Environmental effects testing guidelines.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

o-phenylenediamine

EC Number:

202-430-6

EC Name:

o-phenylenediamine

Cas Number:

95-54-5

Molecular formula:

C6H8N2

IUPAC Name:

benzene-1,2-diamine

Details on test material:

- Purity: 99.8%

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Solutions for quantitative analysis were taken from controls and selected test solutions at 0, 7, 14, and 21 days of the study. Two replicates per concentration were selected using random numbers on each sample day.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Nominal and measured concentrations:

Nominal: 0.0066, 0.016, 0.041, 0.1, 0.26, 0.64, and 1.6 mg/L
Measured: Lowest concentration was below detection limit, 0.0027, 0.021, 0.065, 0.18, 0.54, and 1.5 mg/L, respectively

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: Pyrex beakers 250 mL; 6.1 cm diameter
- Aeration: Unaerated
- No. of organisms per vessel: 4
- No. of vessels per concentration (replicates): 10

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.065 mg/L

Nominal / measured:

meas. (not specified)

Basis for effect:

reproduction

Effect concentrations 2

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

0.54 mg/L

Nominal / measured:

meas. (not specified)

Basis for effect:

mortality

Effect concentrations 3

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

0.18 mg/L

Nominal / measured:

meas. (not specified)

Basis for effect:

reproduction

Effect concentrations 4

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

0.67 mg/L

Nominal / measured:

meas. (not specified)

Basis for effect:

mortality

Remarks on result:

other: 0.57-0.83 = 95% fiducial interval

Effect concentrations 5

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

0.46 mg/L

Nominal / measured:

meas. (not specified)

Basis for effect:

reproduction

Remarks on result:

other: 0.33-0.77 = 95% fiducial interval

Reported statistics and error estimates:

For length: Jonckherre's trend test and Kruskal-Wallis and Dunn's multiple comparison test.

Any other information on results incl. tables

Mean Measured Concentration (mg/L)	Percent Adult Survival	1^stReprod Day	Total Young	Young/Surv. Parent	Immobilized Young	Length (mm)
Control	93	8.0	399	105	0	4.5
0.0066	88	8.0	340	93	0	4.5
0.0027	95	8.0	355	93	0	4.5
0.021	95	8.1	372	96	0	4.5
0.065	85	8.1	324	93	0	4.4
0.18	95	8.0	319	82	0	4.3
0.54	55	12	64	30	3.5	3.8
1.5	0	D	D	D	D	D

D = all dead

Applicant's summary and conclusion

Conclusions:

This study established a 21-day EC50 (mortality) and EC50 (reproduction) for o-phenylenediamine in daphnids of 0.67 and 0.46 mg/L, respectively. The NOEC established by this study was 0.065 mg/L o-phenylenediamine.

Executive summary:

Nominal test concentrations of oPDA were 0.0066, 0.016, 0.041, 0.10, 0.26, 0.64 and 1.6 mg/L. The lowest concentration (0.0066 mg/L) was below the present limit of quantitation and was not analyzed. The remaining mean, measured concentrations were 0.0027, 0.021, 0.065, 0.18, 0.54, and 1.5 mg/L, respectively. The number of adults surviving to day 21 was statistically significantly reduced at 0.54 mg/L and above. The total number of young and the total number of young produced per surviving adult in 21 days was statistically significantly reduced at 0.18 mg/L and above. Length was statistically significantly reduced at 0.065 mg/L and above using Jonckheere's trend test. However, length at 0.065 mg/L was not statistically significant using Kruskal-Wallis and Dunn's multiple comparison test. In addition, length at 0.065 mg/L was not considered biologically significant based on the slight decrease in average measurement compared to control, the variability between replicates, and the lack of precision in measuring daphnid length. The 21-day EC50, based on adult survival and nominal concentrations, was 0.67 mg/L. The 21-day EC50, based on the total number of young produced per surviving adult in 21 days and nominal concentrations was 0.46 mg/L. The 21-day NOEC was 0.065 mg/L and 21 -day MATC was 0.11 mg/L, based upon adult reproduction and length.