Registration Dossier
Registration Dossier
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EC number: 202-430-6 | CAS number: 95-54-5
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OTS 797.1400 (Fish Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- other: Bulletin of National Affairs, Inc. Environmental Effects. Section 797-1400. Fish acute toxicity test. 12 July 1987. 31:6201.
- GLP compliance:
- yes
Test material
- Reference substance name:
- o-phenylenediamine
- EC Number:
- 202-430-6
- EC Name:
- o-phenylenediamine
- Cas Number:
- 95-54-5
- Molecular formula:
- C6H8N2
- IUPAC Name:
- benzene-1,2-diamine
- Details on test material:
- -Purity: 99.8%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Nominal and measured concentrations:
- Nominal: 0, 6.25, 12.5, 25, 50, 100 and 200 mg/L
Measured: 0, 6.8, 13, 28, 51, 98, 210 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: Open
- Material, size, headspace, fill volume: exposure chambers: 20.5 (length) x 21 (width) x 26 (height) cm; 18 cm depth.
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): Volume of each replicate was exchanged seven times daily.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 42.9 mg/L
- Nominal / measured:
- meas. (not specified)
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 35.4-52.5 mg/L
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Cumulative Mortality in Percent
Mean Measured Concentration (mg/L)
24 Hours
48 Hours
72 Hours
96 Hours
A
B
A
B
A
B
A
B
Control
0
0
0
0
0
0
0
0
6.8
0
0
0
0
0
0
0
0
13
0
0
0
0
0
0
0
10
28
0
0
0
10
0
10
10
10
51
10
10
30
40
40
80
40
80
98
100
100
100
100
100
100
100
100
210
100
100
100
100
100
100
100
100
Applicant's summary and conclusion
- Conclusions:
- This study established a 96 hour LC50 for o-phenylenediamine in rainbow trout fingerlings of 42.9 mg/L.
- Executive summary:
oPDA was tested in a 96-hour, flow-through test using fingerling rainbow trout with measured concentrations of 6.8, 13, 28, 51, 98, and 210 mg/L. All chemical and physical parameters were within expected ranges. The 96-hour LC50 was 42.9 mg/L.
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