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EC number: 225-625-8 | CAS number: 4979-32-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data from peer-reviewed handbook or collection of data, peer-reviewed in OECD-SIDS (2004)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- (1984)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- yes
- Details on test solutions:
- - Dilution Water Source: Elendt M4 recommended by OECD TG 211 was used as dilution water.
- Dimethylformamide was used as solvent. 100uL/L of dimethylformamide was contained in the each test soltion.
- Stock Solutions Preparations and Stability: Test chemical was refrigerated. A 0.04mg test chemical in a solvent was dissolved in 1,000mL dilution water. Infrared absorption spectrum of the refrigerated test chemical was detected at the start and the end of the test, and both spectrums are not conradictory to each other. Solvent was prepared as 40mg/10mL of dimethylformamide diluted to 10 times by dimethylformamide. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Age: < 24 hours old
- Supplier/Source: Test organisms were obtained from National Institute for Environmental Studies Japan) and had been reproduced in the testing laboratory for 7 years.
- Any pretreatment: Parental daphnids were acclimated for 28 days on test condition before testing. During acclimination, test daphnids were fed with Chlorella vulgaris, 0.2 mg carbon/day/individual. The mortality of the daphnids was less than 5% for 2 weeks before testing. Any resting-egg and male daphnia was not observed. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20.1 - 20.3ºC
- pH:
- 8.0 ¿ 8.2
- Dissolved oxygen:
- 8.4 - 8.8mg/L
- Nominal and measured concentrations:
- Nominal concentrations: control, solvent control, 0.005, 0.0084, 0.014, 0.024 and 0.04 mg/L
Measured concentrations (after 24 hours exposure): <0.00001, <0.00001, 0,00478, 0,00799, 0.0125, 0.0252, and 0.0385 mg/l
Measured concentrations (after 24 hours exposure): <0.00001, <0.00001, 0.00218, 0.00342, 0.00685, 0.0144, and 0.0256 mg/l
Measured concentrations (geometric mean): <0.00001, <0.00001, 0.00323, 0.00523, 0.00925, 0.0190, and 0.0314 mg/l
The deviation between the nominal and the measured concentrations were less than +/- 20% - Details on test conditions:
- - Exposure Vessel Type: 100 mL test solution in a 100 mL glass beaker
- Number of Replicates: 4
- Individuals per Replicates: 5
- Light Condition: 16:8 hours, light-darkness cycle
- Feeding: None
- Aeration : not described - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.031 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- No test organisms were immobilized at any concentrations tested.
- Results with reference substance (positive control):
- EC50(48hr, immobility) for reference substance (potassium dichromate) was 0.89mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 48-hour EC50 of >0.031 mg/L of DCBS on Daphnia magna was determined according to the OECD Guideline 202 in a semi-static system (MITI, 2001). At the highest concentration (0.0314 mg/L) tested no toxic effect was observed.
- Executive summary:
A 48-hour EC50 of >0.031 mg/L of DCBS on Daphnia magna was determined according to the OECD Guideline 202 in a semi-static system (MITI, 2001). At the highest concentration (0.0314 mg/L) tested no toxic effect was observed.
Reference
The analysis of the test solutions showed that the ratio of the measured values to the nominal value, ±20%, was exceeded, so the geometric mean of the measured values was used to calculate the results. The half-life in purified water was 30 hours (0.04 mg/L) and the main reason for the decrease was considered to be hydrolysis.
Results after 48 h of exposure
mg/L | 95 % confidence level (mgL) | |
Half swimming inhibition concentration (EiC50) | >0.0314 | Calculation not possible |
No observed effective concentration (NOECi) | >0.0314 | - |
Minimum concentration of 100% inhibition | >0.0314 | - |
Description of key information
A 48-hour EC50 of >0.031 mg/l of DCBS on Daphnia magna was determined according to the OECD Guideline 202 in a semi-static system (MITI, 2001). At the highest concentration (0.0314 mg/l) tested no toxic effect was observed.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 0.031 mg/L
Additional information
Should read >0.031 mg/L.
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