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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data from peer-reviewed handbook or collection of data, peer-reviewed in OECD-SIDS (2004)
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
(1984)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
yes
Details on test solutions:
- Dilution Water Source: Elendt M4 recommended by OECD TG 211 was used as dilution water.
- Dimethylformamide was used as solvent. 100uL/L of dimethylformamide was contained in the each test soltion.
- Stock Solutions Preparations and Stability: Test chemical was refrigerated. A 0.04mg test chemical in a solvent was dissolved in 1,000mL dilution water. Infrared absorption spectrum of the refrigerated test chemical was detected at the start and the end of the test, and both spectrums are not conradictory to each other. Solvent was prepared as 40mg/10mL of dimethylformamide diluted to 10 times by dimethylformamide.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Age: < 24 hours old
- Supplier/Source: Test organisms were obtained from National Institute for Environmental Studies Japan) and had been reproduced in the testing laboratory for 7 years.
- Any pretreatment: Parental daphnids were acclimated for 28 days on test condition before testing. During acclimination, test daphnids were fed with Chlorella vulgaris, 0.2 mg carbon/day/individual. The mortality of the daphnids was less than 5% for 2 weeks before testing. Any resting-egg and male daphnia was not observed.
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20.1 - 20.3ºC
pH:
8.0 ¿ 8.2
Dissolved oxygen:
8.4 - 8.8mg/L
Nominal and measured concentrations:
Nominal concentrations: control, solvent control, 0.005, 0.0084, 0.014, 0.024 and 0.04 mg/L
Measured concentrations (after 24 hours exposure): <0.00001, <0.00001, 0,00478, 0,00799, 0.0125, 0.0252, and 0.0385 mg/l
Measured concentrations (after 24 hours exposure): <0.00001, <0.00001, 0.00218, 0.00342, 0.00685, 0.0144, and 0.0256 mg/l
Measured concentrations (geometric mean): <0.00001, <0.00001, 0.00323, 0.00523, 0.00925, 0.0190, and 0.0314 mg/l
The deviation between the nominal and the measured concentrations were less than +/- 20%
Details on test conditions:
- Exposure Vessel Type: 100 mL test solution in a 100 mL glass beaker
- Number of Replicates: 4
- Individuals per Replicates: 5
- Light Condition: 16:8 hours, light-darkness cycle
- Feeding: None
- Aeration : not described
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 0.031 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
No test organisms were immobilized at any concentrations tested.
Results with reference substance (positive control):
EC50(48hr, immobility) for reference substance (potassium dichromate) was 0.89mg/L.

The analysis of the test solutions showed that the ratio of the measured values to the nominal value, ±20%, was exceeded, so the geometric mean of the measured values was used to calculate the results. The half-life in purified water was 30 hours (0.04 mg/L) and the main reason for the decrease was considered to be hydrolysis.


Results after 48 h of exposure

























 mg/L95 % confidence level (mgL)
Half swimming inhibition concentration (EiC50)>0.0314Calculation not possible
No observed effective concentration (NOECi)>0.0314-
Minimum concentration of 100% inhibition>0.0314-
Validity criteria fulfilled:
yes
Conclusions:
A 48-hour EC50 of >0.031 mg/L of DCBS on Daphnia magna was determined according to the OECD Guideline 202 in a semi-static system (MITI, 2001). At the highest concentration (0.0314 mg/L) tested no toxic effect was observed.
Executive summary:

A 48-hour EC50 of >0.031 mg/L of DCBS on Daphnia magna was determined according to the OECD Guideline 202 in a semi-static system (MITI, 2001). At the highest concentration (0.0314 mg/L) tested no toxic effect was observed.

Description of key information

A 48-hour EC50 of >0.031 mg/l of DCBS on Daphnia magna was determined according to the OECD Guideline 202 in a semi-static system (MITI, 2001). At the highest concentration (0.0314 mg/l) tested no toxic effect was observed.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Effect concentration:
0.031 mg/L

Additional information

Should read >0.031 mg/L.